PMID:
Zhonghua Xue Ye Xue Za Zhi. 2019 Jan 14 ;40(1):63-68. PMID: 30704231
Abstract Title:
[Artesunate attenuate chronic graft-versus-host disease by regulating Th17/Treg balance].
Abstract:
To investigate the effects of artesunate treatment on chronic graft-versus-host disease (cGVHD).Recipient BALB/c mice received 8× 10(6) bone marrow cells with 8×10(6) spleen cells from B10D2 mice. Artesunate solubilized in acetone was injected intraperitoneally every day at the dose of 1 mg/kg at Day 28 after BMT. The clinical scores, survival and histopathological damage were analyzed. The frequency of Th17 and Tregs in PB and spleens from the mice were evaluated by flow cytometry. In addition, CD4(+) T cells from the spleens of mice were cultured in vitro, then stimulated with artesunate, the frequency of Th17 and Tregs in these splenocytes were evaluated by flow cytometry.Artesunate administration diminished clinical and histopathological damage, and improved the survival of cGVHD mice[(46.57±7.83)%(55.71±6.99)%,(2)=5.457,=0.020]; Artesunate contributed to Tregs development [(4.45±0.04)%(8.40±0.23)%,=15.679,
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