PMID:
Pharmacognosy Res. 2017 Apr-Jun;9(2):128-132. PMID: 28539735
Abstract Title:
Pharmacognostic Specification, Chlorogenic Acid Content, andAntioxidant Activities ofFlowering Bud.
Abstract:
BACKGROUND: Thunb. or Japanese Honeysuckle has been widely used in traditional medicine for antipyretic.OBJECTIVE: To establish the pharmacognostic specification offlowering bud in Thailand and to determine its chlorogenic acid content andantioxidant activities.MATERIALS AND METHODS: Driedflowering bud from 15 various herbal drugstores throughout Thailand were investigated for pharmacognostic specification. Their chlorogenic acid contents were quantitatively analyzed by thin layer chromatography (TLC) densitometry with winCATS software. The mobile phase for TLC development consisted of ethyl acetate: formic acid: acetic acid: water (10:1.1:1.1:2.6). Antioxidant activities were investigated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, ferric ion reducing antioxidant power assay, nitric oxide scavenging assay, andβ-carotene bleaching assays.RESULTS: Qualifiedflowering bud in Thailand was presented that the contents of loss on drying, total ash, acid-insoluble ash, and water should not be>10.11%, 6.59%, 1.14%, and 10.82% by weight, respectively. The ethanol and water soluble extractive values should not be
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