PMID:
Biomed Pharmacother. 2019 Jun 5 ;117:109058. Epub 2019 Jun 5. PMID: 31176168
Abstract Title:
Cytological effects of honokiol treatment and its potential mechanism of action in non-small cell lung cancer.
Abstract:
PURPOSE: In this study, we aimed to explore key micro(mi)RNAs and their potential regulatory mechanisms induced by honokiol treatment in non-small cell lung cancer (NSCLC) cells.METHODS: NSCLC A549 cells were treated with 0 (control) or 45 μM honokiol. Cell proliferation and migration were determined using CCK-8 and transwell assay, respectively, and apoptosis was determined using flow cytometry. RNA-sequencing was performed to detect the transcript expression levels. The differentially expressed miRNAs (DE-miRNAs) between the honokiol group and the control group were screened and analyzed for their functions and pathways. Then, protein-protein interaction (PPI) networks and miRNA-mRNA regulatory networks were constructed. In addition, survival analysis based on the key miRNAs was performed. Finally, the expression of the key miRNAs and their target genes were determined, and their effects on drug sensitivity were validated using their inhibitors.RESULTS: Cell proliferation and migration were inhibited (P
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