PMID:
Cell Tissue Res. 2018 Dec ;374(3):629-641. Epub 2018 Jul 21. PMID: 30032437
Abstract Title:
Sulforaphane protects granulosa cells against oxidative stress via activation of NRF2-ARE pathway.
Abstract:
Sulforaphane (SFN) has been considered as an indirect antioxidant and potential inducer of the Nrf2-ARE pathway. This study was conducted to investigate the protective role of SFN against oxidative stress in bovine granulosa cells (GCs). GCs were collected from antral follicles (4-8 mm) and cultured according to the experimental design where group 1 = control, group 2 = treated with SFN, group 3 = treated with hydrogen peroxide (HO), group 4 = pretreated with SFN and then with HO(protective) and group 5 = treated with HOfollowed by SFN treatment (rescuing). Results showed that SFN pretreatment significantly increases cell viability and reduces cytotoxicity in GCs under oxidative stress. Following HOexposure, expression of NRF2 was found to be significantly increased (p
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