PMID:
Arch Toxicol. 2019 Jul 30. Epub 2019 Jul 30. PMID: 31363819
Abstract Title:
Aluminum affects neural phenotype determination of embryonic neural progenitor cells.
Abstract:
Aluminum (Al) is a neurotoxin and is associated with the etiology of neurodegenerative diseases, such as Alzheimer's disease (AD). The Al-free ion (Al) is the biologically reactive and toxic form. However, the underlying mechanisms of Al toxicity in the brain remain unclear. Here, we evaluated the effects of Al(in the chloride form-AlCl) at different concentrations (0.1-100 µM) on the morphology, proliferation, apoptosis, migration and differentiation of neural progenitor cells (NPCs) isolated from embryonic telencephalons, cultured as neurospheres. Our results reveal that Alat 100 µM reduced the number and diameter of neurospheres. Cell cycle analysis showed that Alhad a decisive function in proliferation inhibition of NPCs during neural differentiation and induced apoptosis on neurospheres. In addition, 1 µM Alresulted in deleterious effects on neural phenotype determination. Flow cytometry and immunocytochemistry analysis showed that Alpromoted a decrease in immature neuronal markerβ3-tubulin expression and an increase in co-expression of the NPC marker nestin and glial fibrillary acidic protein. Thus, our findings indicate that Alcaused cellular damage and reduced proliferation and migration, resulting in global inhibition of NPC differentiation and neurogenesis.
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