PMID:
Nutr Cancer. 2019 ;71(3):461-471. Epub 2018 Oct 29. PMID: 30372130
Abstract Title:
Blackberry Extract Inhibits Telomerase Activity in Human Colorectal Cancer Cells.
Abstract:
Several mechanisms have been proposed to explain berries' anticancer effects. However, no previously published studies have investigated berries' anti-telomerase activity. In this study, the anti-telomerase activity of blackberry crude extract was analyzed in six human colorectal cancer (CRC) cell lines by TRAP assay. The peripheral blood mononuclear cells (PBMCs) from a healthy donor were used as a normal control. We also examined the effect of blackberry on the human telomerase RNA (hTR) mRNA level and on human telomerase reverse transcriptase (hTERT) expression and promoter methylation in CRC cells. Blackberry extract significantly inhibited the growth of six CRC cell lines in a dose-dependent manner. Telomerase activity of CRC cells incubated with the ICconcentration of berry's extract for 48 and 72 h decreased by 15%-37.5% and 43.23%-62.5% (P
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