PMID:
Front Microbiol. 2017 ;8:625. Epub 2017 Apr 21. PMID: 28484429
Abstract Title:
Protective Effect of Carvacrol against Gut Dysbiosis andAssociated Disease in a Mouse Model.
Abstract:
This study investigated the effect of carvacrol (CR), a phytophenolic compound on antibiotic-associated gut dysbiosis andinfection in a mouse model. Five to six-week-old C57BL/6 mice were randomly divided into seven treatment groups (challenge and control) of eight mice each. Mice were fed with irradiated feed supplemented with CR (0, 0.05, and 0.1%); the challenge groups were made susceptible toby orally administering an antibiotic cocktail in water and an intra-peritoneal injection of clindamycin. Both challenge and control groups were infected with 10CFU/ml of hypervirulent(ATCC 1870) spores or PBS, and observed for clinical signs for 10 days. Respective control groups for CR, antibiotics, and their combination were included for investigating their effect on mouse enteric microflora. Mouse body weight and clinical and diarrhea scores were recorded daily post infection. Fecal samples were collected for microbiome analysis using rRNA sequencing in MiSeq platform. Carvacrol supplementation significantly reduced the incidence of diarrhea and improved the clinical and diarrhea scores in mice (
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