PMID:
Zhejiang Da Xue Xue Bao Yi Xue Ban. 2019 May 25 ;48(3):296-302. PMID: 31496162
Abstract Title:
[High dose vitamin C inhibits proliferation of breast cancer cells through reducing glycolysis and protein synthesis].
Abstract:
OBJECTIVE: To investigate the effects of high dose vitamin C (VC) on proliferation of breast cancer cells and to explore its mechanisms.METHODS: Human breast cancer cells Bcap37 and MDA-MB-453 were treated with VC at low dose (0.01 mmol/L), medium dose (0.10 mmol/L) and high dose (2.00 mmol/L). Cell proliferation was determined with CCK-8 assay, protein expression was evaluated by Western blot, and the secretion of lactic acid in tumor cells was detected by colorimetric method. Bcap37 cells were inoculated in nude mice, and tumor baring nude mice were intraperitoneally injected with high VC(4 g/kg, VC group,=5)or normal saline (control group,=5) for 24 d. Tumor weight and body weight were calculated.RESULTS: experiments demonstrated that high dose VC significantly inhibited cell proliferation in Bcap37 and MDA-MB-453 cells (all
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