PMID:
Afr J Infect Dis. 2018 ;12(1 Suppl):36-43. Epub 2018 Mar 7. PMID: 29619428
Abstract Title:
ANTIVIRAL ACTIVITY OFBurm.f. LEAVES AGAINST HIV-INFECTED MT-4 CELLS.
Abstract:
Backgrounds: Burm.f. has been known to have anti-HIV activity. This study was conducted to evaluate the effect of incubation time on the antiviral activity of theleaves extract on HIV-infected MT-4 cells. Molecular docking test was also conducted to determine the interaction of alkaloids and flavonoids on theleaves against HIV-1 reverse transcriptase receptor. It is expected that this research will provide scientific information on the development ofleaves as an anti-HIV drug.Materials and Methods: In the activity test, the effect of incubation time on the antiviral activity ofleaves on HIV-infected MT-4 cells were evaluated. During the activity test, a parameter of cytolysis effect inhibition on MT-4 cell line was observed after 4 days and 6 days incubation period. The molecular docking test is performed by using Molegro Virtual Docker software to determine the interaction of alkaloid and flavonoid compounds ofleaves with HIV-1 reverse transcriptase receptor.Results: The incubation time influences the CCand ECvalue. Fractionated-70% ethanol extract ofleaves showed a higher anti-HIV activity with EC= 3.045 x 10μg/mL, SI = 6.309 x 10(4 days of incubation) and EC= 6.066μg/mL, SI = 58494.845 (6 days of incubation). From molecular docking test, it was found that flavonoid ofleaves could inhibit the activity of HIV reverse transcriptase enzyme.Conclusion: Fractionated-70% ethanol extract ofhas potential as an anti-HIV.
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