PMID:
J Clin Endocrinol Metab. 2019 Sep 19. Epub 2019 Sep 19. PMID: 31536135
Abstract Title:
In utero bisphenol A exposure is linked with sex specific changes in the transcriptome and methylome of human amniocytes.
Abstract:
CONTEXT: Prenatal exposure to bisphenol A (BPA) is linked to obesity and diabetes, but the molecular mechanisms driving these phenomena are not known. Alterations in DNA methylation in amniocytes exposed to BPA in utero represent a potential mechanism leading to metabolic dysfunction later in life.OBJECTIVE: To profile changes in genome-wide DNA methylation and expression in second trimester human amniocytes exposed to BPA in utero.DESIGN: A nested case-control study was performed in amniocytes matched for offspring sex, maternal race/ethnicity, maternal age, gestational age at amniocentesis and gestational age at birth. Cases had amniotic fluid BPA measuring 0.251-23.74 ng/mL. Sex-specific genome-wide DNA methylation analysis and RNA-sequencing were performed to determine differentially methylation regions (DMRs) and gene expression changes associated with BPA exposure. Ingenuity pathway analysis was performed to identify biologically relevant pathways enriched after BPA exposure. In silico Hi-C analysis identified potential chromatin interactions with DMRs.RESULTS: There were 101 genes with altered expression in male amniocytes exposed to BPA (q
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