Vitamin C may exert variable effects on viability and proliferation of HeLa cells exhibiting high and low chromosomal instability.

PMID: 

Adv Clin Exp Med. 2019 01 ;28(1):19-24. PMID: 30156389

Abstract Title: 

Vitamin C may exert variable effects on viability and proliferation of HeLa cells exhibiting high and low chromosomal instability.

Abstract: 

BACKGROUND: Chromosomal instability (CIN), defined as abnormality in chromosome structure or number, is the hallmark of malignancies. The role of vitamin C in cancer treatment is controversial and its effect on CIN is still an open field of research. In this work, we tried to study the effect of high-dose L-ascorbic acid (L-AA) on CIN-induced (CINhi = CIN high) and non-CIN-induced (CINlo = CIN low) cervical cancer cells.OBJECTIVES: The aim of this study was to explore the effect of high-dose L-AA on CIN in the cervical cancer cell line (HeLa) cells.MATERIAL AND METHODS: The HeLa cells (CINhi and CINlo) were treated with 2 doses (5 mM and 8 mM) of L-AA for 24 h and 48 h. They were then analyzed by micronucleus (MN) scoring, cell ploidy and flow cytometry, the latter regardingγH2AX expression. Cell viability was assessed by the methylthiazol tetrazolium (MTT) and Annexin V assays.RESULTS: Treatment of CINhi cells with L-AA led to a decrease in MN score (colchicine – 71.5±4.95, 67.5 ±0.71; L-AA (5 mM) – 49 ±7.07, 46.5 ±4.95; L-AA (8 mM) – 42 ±9.89, 41 ±1.41, at 24 h and 48 h, respectively; p

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