PMID:
Balkan Med J. 2019 Oct 9. Epub 2019 Oct 9. PMID: 31594288
Abstract Title:
Inhibition of Lung Cancer Proliferation by Wogonin is Associated with the Activation of Apoptosis and Generation of Reactive Oxygen Species
Abstract:
Background: Lung cancer has very high incidence rate and is one among the commonly diagnosed tumors in the developed countries.Aims: The present study was designed to investigate the effect of wogonin on A549 and A427 lung cancer cells and explore the mechanism involved.Study Design: In the present study effect of wogonin was investigatedon A549 and A427 lung cancer and BEAS-2B normal cell normal lung cells.Methods: The cytotoxicity effect of wogoninon A549 and A427 lung cancer and BEAS-2B cells was assessed by MTT assay. The apoptosis onset was assessed by flow cytometry using Annexin V FITC/PI staining. Western blotting was used for determination of changes in apoptotic protein expression.Results: Wogonin treatment exhibited cytotoxicity effect selectively on A549 and A427 cells without affecting BEAS-2B normal lung cells. The viability of A549 and A427 cells was reduced to 31 and 34%, respectively on treatment with 50µM of wogonin. There was no significant reduction in BEAS-2B cell viability on treatment with 50 µM of wogonin. The percentage of apoptotic A427 cells showed a significant (P
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