PMID:
Life Sci. 2019 Oct 9 ;236:116948. Epub 2019 Oct 9. PMID: 31605711
Abstract Title:
Ginsenoside Rg1 defenses PC-12 cells against hydrogen peroxide-caused damage via up-regulation of miR-216a-5p.
Abstract:
BACKGROUND: Spinal cord injury (SCI) is a destructive trauma accompanied with local injury. Ginsenoside Rg1 exerts anti-apoptosis and anti-autophagy properties. Our goal was to study the protective mechanism of Rg1 in attenuating cell injury.METHODS: MiR-216a-5p inhibitor was transfected into PC-12 cells to verify the growth promoting roles of miR-216a-5p, then cells were pre-treated by Rg1 for 24 h and treated by 300 μM hydrogen peroxide (HO) for 1 h. Cell viability and apoptosis were tested through Cell Counting Kit-8 (CCK-8) and flow cytometry, respectively. Expression of miR-216a-5p and cell damage relative factors was tested via qRT-PCR and Western blot experiments.RESULTS: HOinduced cell activity suppression, apoptosis and clear autophagy well at the concentration of 300 μM Rg1 attenuated HO-induced cell injury at the concentration of 200 μM that it elevated cell activity, attenuated apoptosis and autophagy and activated phosphatidylinositol 3 kinase (PI3K)/AMP-activated protein kinase (AKT) and AMP-activated protein kinase (AMPK) signal pathways. Further, miR-216a-5p was up-regulated by Rg1.CONCLUSION: Our study demonstrated that Rg1 attenuated HO-caused cell injury through positively regulated miR-216a-5p.
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