PMID:
Biomed Rep. 2019 Nov ;11(5):207-214. Epub 2019 Sep 10. PMID: 31632668
Abstract Title:
Protective functions of Lycium barbarum polysaccharides in HO-injured vascular endothelial cells through anti-oxidation and anti-apoptosis effects.
Abstract:
Cell injury in the cardiovascular endothelia caused by oxidative stress is among the major inducers of endothelium dysfunction and serves an important role in initiating cardiovascular diseases (CVDs). Therefore, protecting and improving the normal function of endothelial cells are considered key measures against CVDs. As a traditional Chinese medicinal component,is regarded to have high medicinal value. The present study aimed to investigate the potential anti-apoptosis and anti-oxidation effects ofpolysaccharides (LBPs) on injured rat artery endothelial cells, to demonstrate the experimental and medicinal values of LBPs. In the present study, the aortic endothelial cells of rats were cultivated and randomly divided into five groups: A control group, HO-injured group (HOgroup), HO+LBPs (110µg/ml) group (low-dose group, LT), HO+LBPs (220µg/ml) group (medium-dose group, MT) and HO+LBPs (440µg/ml) group (high-dose group, HT). Among these, the activity of superoxide dismutase (SOD), and the levels of malondialdehyde (MDA) and nitric oxide (NO) were detected by colorimetry. Additionally, the expression of B-cell lymphoma-2 (Bcl-2) and Bcl-2-associated X protein (Bax) were detected by western blotting. It was observed that SOD activity and NO content decreased while MDA content increased significantly in the HOgroup (P
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