PMID:
Int J Ophthalmol. 2019 ;12(10):1517-1523. Epub 2019 Oct 18. PMID: 31637185
Abstract Title:
Celastrol inhibits migration, proliferation and transforming growth factor-β2-induced epithelial-mesenchymal transition in lens epithelial cells.
Abstract:
AIM: To investigate the mechanism of celastrol in inhibiting lens epithelial cells (LECs) fibrosis, which is the pathological basis of cataract.METHODS: Human LEC line SRA01/04 was treated with celastrol and transforming growth factor-β2 (TGF-β2). Wound-healing assay, proliferation assay, flow cytometry, real-time polymerase chain reaction (PCR), Western blot and immunocytochemical staining were used to detect the pathological changes of celastrol on LECs. Then, we cultured Sprague-Dawley rat lens in medium as a semi-model to find the function of celastrol further.RESULTS: We found that celastrol inhibited the migration of LECs, as well as proliferation (
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