PMID:
J Biosci Bioeng. 2019 Oct 17. Epub 2019 Oct 17. PMID: 31630942
Abstract Title:
Novel anti-obesity peptide (RLLPH) derived from hazelnut protein hydrolysates inhibits adipogenesis in 3T3-L1 adipocytes by regulating adipogenic transcription factors and adenosine monophosphate-activated protein kinase (AMPK) activation.
Abstract:
Hazelnut proteins are an excellent source of bioactive peptides. Our previous study demonstrated that several novel peptides derived from Corylus heterophylla Fisch (C. heterophylla Fisch) have antioxidant and anti-inflammatory activities. In this study, we purified and identified anti-obesity peptides from hazelnut protein hydrolysates by chromatography and liquid chromatography-tandem mass spectrometry (LC-MS/MS). Subsequently, we evaluated the inhibitory effect of the synthetic peptide on adipogenesis in 3T3-L1 adipocytes by Oil Red O staining, reverse transcription polymerase chain reaction (RT-PCR) and western blot. The results showed that a novel synthetic pentapeptide, Arg-Leu-Leu-Pro-His (RLLPH), derived from the C3 fraction, attenuated adipogenesisby downregulating the expression of several mRNAs related to adipogenesis, including peroxisome proliferator-activated receptor gamma (PPARγ), CCAAT/enhancer binding protein alpha (C/EBPα), adipocyte fatty acid-binding protein 2 (aP2), sterol regulatory element binding protein 1c (SREBP-1c), fatty acid synthase (FAS), acetyl-CoA carboxylase 1 (ACC1), and 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR). Moreover, RLLPH upregulated the levels of phosphorylated Adenosine monophosphate-activated protein kinase (AMPK) and its substrate acetyl-CoA carboxylase (ACC) in 3T3-L1 adipocytes. A stability study revealed that RLLPH was relatively stable during pepsin digestion. These findings suggest that RLLPH has potential anti-obesity effects and may help combat metabolic diseases.
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