PMID:
Mol Nutr Food Res. 2019 Dec 14:e1901017. Epub 2019 Dec 14. PMID: 31837654
Abstract Title:
Neuroprotective Effects of apple-derived Drinks in a Mice Model of Inflammation.
Abstract:
SCOPE: Fruit-derived drinks consumption is considered beneficial due to the antioxidant and neuroprotective effects of polyphenols separately, but studies including their total constituents are scarce. In this work, we determined the antioxidant and anti-inflammatory neuroprotective effects of apple-derived beverages in a mouse model of LPS-induced inflammation.METHODS AND RESULTS: Preliminary antioxidant and neuroinflammatory experiments were carried out with fifteen drink polyphenolic extracts in SH-SY5Y and BV2 cells, using HOas pro-oxidant and LPS as pro-inflammatory stimulus, respectively. Extracts improved antioxidant systems functioning and presented neuroprotective mitochondrial-related effects. In microglia, extracts reduced reactive oxygen species and modulated cytokine release. To better mimic human consumption, four concentrated dealcoholized apple-derived drinks (three ciders and apple juice) were supplied to mice for seven days in substitution of drinking water. Mice treated with beverages presented reduced brain oxidative stress and inflammatory markers (lipid peroxidation, NO, iNOS, TNF-α) after LPS injection. Interestingly, genetic expression of antioxidant enzymes and glutathione levels were also greatly augmented after drink intake.CONCLUSION: Our results confirm the antioxidant and anti-inflammatory-mediated neuroprotective properties of apple-derived drinks, suggesting that their consumption could be a good approach for prevention of neurodegenerative disorders. To our knowledge, this is the first description of cider neuroprotective effects. This article is protected by copyright. All rights reserved.
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