PMID:
Nan Fang Yi Ke Da Xue Xue Bao. 2019 Oct 30 ;39(10):1180-1185. PMID: 31801717
Abstract Title:
[Effect of Atractylodes macrocephala polysaccharide on proliferation and invasion of hepatocellular carcinoma cells].
Abstract:
OBJECTIVE: To investigate the inhibitory effect of polysaccharide of Atractylodes macrocephala (PAM) on the proliferation and invasion of hepatocellular carcinoma cells and the underlying mechanism.METHODS: Hepatocellular carcinoma HepG2 cells were treated with different concentrations of PAM, and their proliferation and invasive ability were examined using CCK-8 assay and Transwell assay. Immunofluorescence assay was performed to detect the expression level ofβ-catenin, and real-time PCR and Western blotting were used to detect the mRNA and protein expressions of AKT, GSK-3β and MMP-2 in the cells. The changes in the proliferation, invasiveness and the expressions of pGSK-3β and MMP2 were examined in the cells following treatment with LiCl/PAM/LiCl plus PAM.RESULTS: PAM treatment significantly reduced the cell viability, the number of migration cells, and the expression levels ofβ-catenin and MMP-2 (
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