PMID:
Molecules. 2019 Nov 18 ;24(22). Epub 2019 Nov 18. PMID: 31752124
Abstract Title:
Anti-Acetylcholinesterase Activities of Mono-Herbal Extracts and Exhibited Synergistic Effects of the Phytoconstituents: A Biochemical and Computational Study.
Abstract:
Alzheimer's disease (AD), a neurodegenerative disease, is the most common form of dementia. Inhibition of acetylcholinesterase (AChE) is a common strategy for the treatment of AD. In this study, aqueous, hydro-methanolic, and methanolic extracts of five potent herbal extracts were tested for their in vitro anti-AChE activity. Among all, the(Giloy) methanolic fraction performed better with an ICof 202.64µg/mL. Of the HPLC analyzed components of(methanolic extract), palmatine and berberine performed better (IC0.66 and 0.94µg/mL, respectively) as compared to gallic acid and the tool compound"galantamine hydrobromide"(IC7.89 and 1.45µg/mL, respectively). Mode of inhibition of palmatine and berberine was non-competitive, while the mode was competitive for the tool compound. Combinations of individual alkaloids palmatine and berberine resulted in a synergistic effect for AChE inhibition. Therefore, the AChE inhibition by the methanolic extract ofwas probably due to the synergism of the isoquinoline alkaloids. Upon molecular docking, it was observed that palmatine and berberine preferred the peripheral anionic site (PAS) of AChE, withπ-interactions to PAS residue Trp286, indicating that it may hinder the substrate binding by partially blocking the entrance of the gorge of the active site or the product release.
Health Time 