PMID:
World J Gastroenterol. 2018 Feb 14 ;24(6):693-705. PMID: 29456408
Abstract Title:
Inhibitory effects of patchouli alcohol on stress-induced diarrhea-predominant irritable bowel syndrome.
Abstract:
AIM: To elucidate the mechanism of patchouli alcohol (PA) in treatment of rat models of diarrhea-predominant irritable bowel syndrome (IBS-D).METHODS: We studied the effects of PA on colonic spontaneous motility using its cumulative log concentration (3× 10mol/L to 1× 10mol/L). We then determined the responses of the proximal and distal colon segments of rats to the following stimuli: (1) carbachol (1× 10mol/L to 1× 10mol/L); (2) neurotransmitter antagonists including N-nitro-L-arginine methyl ester hydrochloride (10μmol/L) and (1R*, 2S*)-4-[2-Iodo-6-(methylamino)-9H-purin-9-yl]-2-(phosphonooxy)bicyclo[3.1.0]hexane-1-methanol dihydrogen phosphate ester tetraammonium salt (1 μmol/L); (3) agonist α,β-methyleneadenosine 5'-triphosphate trisodium salt (100 μmol/L); and (4) single KCl doses (120 mmol/L). The effects of blockers against antagonist responses were also assessed by pretreatment with PA (100 μmol/L) for 1 min. Electrical-field stimulation (40 V, 2-30 Hz, 0.5 ms pulse duration, and 10 s) was performed to observe nonadrenergic, noncholinergic neurotransmitter release in IBS-D rat colon. The ATPlevel of Kreb's solution was also determined.RESULTS: PA exerted a concentration-dependent inhibitory effect on the spontaneous contraction of the colonic longitudinal smooth muscle, and the half maximal effective concentration (EC) was 41.9μmol/L. In comparison with the KCl-treated IBS-D group, the contractile response (mg contractions) in the PA + KCl-treated IBS-D group (11.87 ± 3.34) was significantly decreased in the peak tension (
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