PMID:
Oncol Lett. 2019 Oct ;18(4):4253-4261. Epub 2019 Aug 14. PMID: 31579089
Abstract Title:
Sulforaphane enhances apoptosis induced bystrain S-PT84 via the TNFα pathway in human colon cancer cells.
Abstract:
Sulforaphane andinduce apoptosis in several cancer cells. Sulforaphane, a dietary isothiocyanate, is an attractive agent due to its potent anticancer effects. Sulforaphane suppresses the proliferation of various cancer cellsand. The present study investigated the effect of sulforaphane and a co-culture with-treated peripheral blood mononuclear cells (PBMCs) in human colon cancer cells. The combination markedly induced apoptosis in human colon cancer HCT116 and SW480 cells. A pan-caspase inhibitor markedly inhibited apoptosis, and a tumor necrosis factor (TNF) receptor/Fc chimera partially inhibited apoptosis in both cells. The amount of TNFα secretion in the culture supernatant was significantly increased by co-culture with-treated normal human PBMCs. On the other hand, the expression of cellular inhibitor of apoptosis-2 (cIAP-2), an anti-apoptotic protein, was increased by co-culture with-treated PBMCs in colon cancer cells, but sulforaphane treatment significantly suppressed the induction of cIAP-2. The present results revealed that sulforaphane enhances apoptosis in human colon cancer cells under co-culture with-treated PBMCs via the TNFα signaling pathway.
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