Ascorbic acid induces osteoblast differentiation of human suspension mononuclear cells.

PMID: 

Cytotherapy. 2014 May ;16(5):674-82. Epub 2013 Oct 29. PMID: 24176546

Abstract Title: 

Ascorbic acid induces osteoblast differentiation of human suspension mononuclear cells.

Abstract: 

BACKGROUND AIMS: Suspension mononuclear cells (MNCs) can be differentiated into osteoblasts with the induction of ascorbic acid andβ-glycerophosphate. The aim of this study was to determine the ability of suspension MNCs to differentiate into osteoblasts using ascorbic acid only.METHODS: Suspension MNCs were obtained by a combination of gradient centrifugation and culture selection. Suspension MNCs were subjected to differentiation assay by culturing them inside proliferation medium supplemented with 10μg/mL, 30 μg/mL, 50 μg/mL, 60 μg/mL, 90 μg/mL and 500 μg/mL of ascorbic acid. Proliferation medium supplemented with 50 μg/mL ascorbic acid and 10 mmol/L β-glycerophosphate was used as a positive control for osteoblast induction, and proliferation medium without ascorbic acid was used as anegative control. Differentiation analysis was performed using alkaline phosphatase (ALP) assay, von Kossa staining and expression of osteoblast-related genes.RESULTS: With all concentrations of ascorbic acid used, there was a significant increase (P 

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