PMID:
Gene. 2020 Apr 3:144649. Epub 2020 Apr 3. PMID: 32251702
Abstract Title:
Vitamin D Metabolites Influences Expression Of Genes Concerning Cellular Viability And Function In Insulin Producingβ-Cells (INS1E).
Abstract:
BACKGROUND: Studies have shown that vitamin D can enhance glucose-stimulated insulin secretion (GSIS) and change the expression of genes in pancreaticβ-cells. Still the mechanisms linking vitamin D and GSIS are unknown.MATERIAL AND METHODS: We used an establishedβ-cell line, INS1E. INS1E cells were pre-treated with 10 nM 1,25(OH)vitamin D or 10 nM 25(OH)vitamin D for 72 hours and stimulated with 22 mM glucose for 60 minutes. RNA was extracted for gene expression analysis.RESULTS: Expression of genes affecting viability, apoptosis and GSIS changed after pre-treatment with both 1,25(OH)vitamin D and 25(OH)vitamin D in INS1E cells. Stimulation with glucose after pre-treatment of INS1E cells with 1,25(OH)vitamin D resulted in 181 differentially expressed genes, whereas 526 genes were differentially expressed after pre-treatment with 25(OH)vitamin D.CONCLUSION: Vitamin D metabolites may affect pancreaticβ-cells and GSIS through changed gene expression for genes involved in β-cell function and viability.
Health Time 