PMID:
Eur J Nutr. 2016 Feb ;55(1):171-82. Epub 2015 Jan 17. PMID: 25595100
Abstract Title:
Anthocyanins and phenolic acids from a wild blueberry (Vaccinium angustifolium) powder counteract lipid accumulation in THP-1-derived macrophages.
Abstract:
PURPOSE: Blueberries are a rich source of anthocyanins (ACNs) and phenolic acids (PA), which are hypothesized to protect against development of atherosclerosis. The present study examined the effect of an ACN- and PA-rich fractions, obtained from a wild blueberry powder, on the capacity to counteract lipid accumulation in macrophages derived from monocytic THP-1 cells. In addition, we tested the capacity of pure ACNs and their metabolites to alter lipid accumulation.METHODS: THP-1-derived macrophages were incubated with fatty acids (500μM oleic/palmitic acid, 2:1 ratio) and different concentrations (from 0.05 to 10 μg mL(-1)) of ACN- and PA-rich fractions, pure ACN standards (malvidin, delphinidin and cyanidin 3-glucoside), and metabolites (syringic, gallic and protocatechuic acids). Lipid accumulation was quantified with the fluorescent dye Nile red.RESULTS: Lipid accumulation was reduced at all concentrations of the ACN-rich fraction tested with a maximum reduction at 10μg mL(-1) (-27.4%; p
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