PMID:
World J Gastroenterol. 2020 Apr 14 ;26(14):1601-1612. PMID: 32327909
Abstract Title:
Silymarin, boswellic acid and curcumin enriched dietetic formulation reduces the growth of inherited intestinal polyps in an animal model.
Abstract:
BACKGROUND: Some substances of plant origin have been reported to exert an effect in reducing intestinal neoplasm development, especially in animal models. Adenomatous polyposis coli multiple intestinal neoplasia – Apcis the most studied murine model of genetic intestinal carcinogenesis.AIM: To assess whether an enriched nutritional formulation (silymarin, boswellic acid and curcumin) with provenandanti-carcinogenetic properties may prevent inherited intestinal cancer in animal model.METHODS: Forty adenomatous polyposis coli multiple intestinal neoplasia – Apcmice were used for the study of cancer prevention. They were divided into two groups: 20 assumed standard and 20 enriched diet. At the 110d animals were sacrificed. In each group, four subgroups received intraperitoneal bromodeoxyuridine injection at different times (24, 48, 72 and 96 h before the sacrifice) in order to assess epithelial turnover. Moreover, we evaluated the following parameters: Intestinal polypoid lesion number and size on autoptic tissue, dysplasia and neoplasia areas by histological examination of the whole small intestine, inflammation by histology and cytokine mRNA expression by real-time polymerase chain reaction, bromodeoxyuridine and TUNEL immuno-fluorescence for epithelial turnover and apoptosis, respectively. Additionally, we performed western blotting analysis for the expression of estrogen alpha and beta receptors, cyclin D1 and cleaved caspase 3 in normal and polypoid tissues.RESULTS: Compared to standard, enriched diet reduced the total number (203416) and the mean± SD/animal (12.6 ± 5.026.0± 8.8;
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