PMID: 

Clin Exp Reprod Med. 2019 Aug 14. Epub 2019 Aug 14. PMID: 31408928

Abstract Title: 

The impact of two doses of coenzyme Q10 on semen parameters and antioxidant status in men with idiopathic oligoasthenoteratospermia.

Abstract: 

Objective: Oxidative stress contributes to male infertility, and antioxidants have been recommended for treating idiopathic oligoasthenoteratospermia (OAT). There is, however, a lack of agreement on the type, dosing, and use of individual antioxidants or combinations thereof. The purpose of this study was to compare the effects of two doses of coenzyme Q10 (CoQ10) on semen parameters and antioxidant status in men with idiopathic OAT.Methods: In this prospective study, patients with idiopathic OAT received 200 mg/day (n = 35) or 400 mg/day (n = 30) of CoQ10 orally for 3 months. All patients underwent semen analysis according to the fifth editions of the World Health Organization criteria. Total antioxidant capacity (TAC), catalase (CAT) activity, and superoxide dismutase (SOD) activity were measured both before and after treatment.Results: Treatment with CoQ10 (200 mg/day or 400 mg/day) resulted in a significant increase in sperm concentration from baseline (8.22± 6.88 to 12.53 ± 8.11 million/mL, p= 0.019; 7.58 ± 5.41 to 12.33 ± 6.1 million/mL, p= 0.002, respectively), progressive motility (16.54% ±9.26% to 22.58% ±10.15%, p=0.011; 14.22% ±12.85% to 26.1% ±14.52%, p= 0.001, respectively), and total motility (25.68% ± 6.41% to 29.96% ± 8.09%, p= 0.016; 23.46% ± 12.59% to 34.82% ± 14.17%, p= 0.001, respectively). CoQ10 therapy also increased TAC (p= 0.009, p= 0.001, respectively), SOD activity (p= 0.004, p= 0.001, respectively), and CAT activity (p= 0.039, p= 0.024, respectively). Furthermore, antioxidant measures correlated significantly with seminal fluid parameters (r = 0.36-0.76).Conclusion: CoQ10 supplementation improved semen parameters and antioxidant status in men with idiopathic OAT, with a greater improvement shown in men who took 400 mg/day than in those who took 200 mg/day.

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Clin Rheumatol. 2019 Aug 7. Epub 2019 Aug 7. PMID: 31392559

Abstract Title: 

Effects of coenzyme Q10 supplementation on matrix metalloproteinases and DAS-28 in patients with rheumatoid arthritis: a randomized, double-blind, placebo-controlled clinical trial.

Abstract: 

OBJECTIVES: This study aimed to assess the effect of CoQ10 supplementation on serum matrix metalloproteinases (MMPs) and clinical parameters in rheumatoid arthritis (RA) patients.METHOD: In this randomized, double-blind, placebo-controlled trial, 54 RA patients who fulfilled the eligibility criteria (18-56 years, diagnosed at least 6 months ago, with DAS-28 > 3.2) were randomly assigned into two groups to receive 100 mg/day CoQ10 (n = 27) or placebo (n = 27) for 2 months. Serum MMP-1 and MMP-3 levels and clinical status using disease activity score in 28 joints (DAS-28) were assessed before and after supplementation. Data were analyzed usingχ2, independent sample t test, paired t test, Wilcoxon, Mann-Whitney, and analysis of covariance.RESULTS: A significant reduction was observed in both CoQ10 and placebo groups in the medians of serum MMP-1 (0.2 to 0.16, P 

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PMID: 

Front Cell Dev Biol. 2019 ;7:128. Epub 2019 Jul 16. PMID: 31380368

Abstract Title: 

Reprogramming the Epigenome With Vitamin C.

Abstract: 

The erasure of epigenetic modifications across the genome of somatic cells is an essential requirement during their reprogramming into induced pluripotent stem cells (iPSCs). Vitamin C plays a pivotal role in remodeling the epigenome by enhancing the activity of Jumonji-C domain-containing histone demethylases (JHDMs) and the ten-eleven translocation (TET) proteins. By maintaining differentiation plasticity in culture, vitamin C also improves the quality of tissue specific stem cells derived from iPSCs that are highly sought after for use in regenerative medicine. The ability of vitamin C to potentiate the activity of histone and DNA demethylating enzymes also has clinical application in the treatment of cancer. Vitamin C deficiency has been widely reported in cancer patients and has recently been shown to accelerate cancer progression in disease models. Therapies involving high-dose vitamin C administration are currently gaining traction in the treatment of epigenetic dysregulation, by targeting aberrant histone and DNA methylation patterns associated with cancer progression.

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PMID: 

Food Nutr Res. 2019 ;63. Epub 2019 Apr 26. PMID: 31073285

Abstract Title: 

Antioxidant, antimicrobial, and antiproliferative activity-based comparative study of peel and flesh polyphenols from.

Abstract: 

Background: Kiwifruit () peel has been always considered as useless because of the harsh taste. To promote the full utilization of kiwifruit resources it is essential to explore the nutritional benefits of kiwifruit peel.Objective: Our studies explored the difference in polyphenolic composition and biological activity including antioxidant, antimicrobial, and antiproliferative activity of the flesh and peel of kiwifruit.Design: Antioxidant activity of the extracted polyphenols of the peel and flesh ofwas checked by 2,2-diphenyl-1-picrylhydrazyl, 2,2'-azino-bis3-ethylbenzothiazoline-6-sulphonic acid (ABTS), hydroxyl ion reduction, and ion chelating ability. Antibacterial activity against,, andand antiproliferative activityHepG2 was tested in a dose- and time-dependent manner. Liquid chromatography/mass spectrometry (LC/MS) chromatogram of the peel and flesh further differentiated the phenolic acid profile.Results: The pericarp of kiwifruit was found to be more abundant in polyphenols and flavonoids than the flesh, with contents of 12.8 mg/g and 2.7 mg/g, respectively. LC/MS analysis revealed that the catachin, quercetin and epigallocatechin content (the main polyphenols in kiwifruit) in the peel was significantly higher than in the flesh (

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Braz J Otorhinolaryngol. 2017 Nov – Dec;83(6):691-696. Epub 2016 Nov 5. PMID: 27865708

Abstract Title: 

Effects of exposure to 2100MHz GSM-like radiofrequency electromagnetic field on auditory system of rats.

Abstract: 

INTRODUCTION: The use of mobile phones has become widespread in recent years. Although beneficial from the communication viewpoint, the electromagnetic fields generated by mobile phones may cause unwanted biological changes in the human body.OBJECTIVE: In this study, we aimed to evaluate the effects of 2100MHz Global System for Mobile communication (GSM-like) electromagnetic field, generated by an electromagnetic fields generator, on the auditory system of rats by using electrophysiological, histopathologic and immunohistochemical methods.METHODS: Fourteen adult Wistar albino rats were included in the study. The rats were divided randomly into two groups of seven rats each. The study group was exposed continuously for 30days to a 2100MHz electromagnetic fields with a signal level (power) of 5.4dBm (3.47mW) to simulate the talk mode on a mobile phone. The control group was not exposed to the aforementioned electromagnetic fields. After 30days, the Auditory Brainstem Responses of both groups were recorded and the rats were sacrificed. The cochlear nuclei were evaluated by histopathologic and immunohistochemical methods.RESULTS: The Auditory Brainstem Responses records of the two groups did not differ significantly. The histopathologic analysis showed increased degeneration signs in the study group (p=0.007). In addition, immunohistochemical analysis revealed increased apoptotic index in the study group compared to that in the control group (p=0.002).CONCLUSION: The results support that long-term exposure to a GSM-like 2100MHz electromagnetic fields causes an increase in neuronal degeneration and apoptosis in the auditory system.

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PMID: 

Nutr Cancer. 2019 Aug 7:1-7. Epub 2019 Aug 7. PMID: 31387399

Abstract Title: 

Kiwifruit and Cancer: An Overview of Biological Evidence.

Abstract: 

Kiwifruit consumption is associated with many favorable health benefits. This article is hence aimed to provide an overview on potential effects of kiwifruit on cancer development or progression. An electronic search was performed in Medline and Web of Science, for identifying original studies exploring the relationship between kiwifruit and cancer development or progression, both in vivo and in vitro. The following keywords were used:"Kiwifruit"OR"Kiwi"OR""OR","in combination with"cancer"OR"malignancy"OR"neoplasm."Overall, 58 items could be retrieved, fifty of which were excluded since they did not fulfilled all our search criteria. Eight studies (five in vitro studies, two human intervention trials, and one both in vivo and in human study) were finally included in this critical literature review. Taken together, the results of these published studies suggest that kiwifruit supplementation may be associated with direct and indirect anticancer effects, the former likely due to ascorbic acid-mediated reduction of DNA oxidative injury and cytotoxic effect on cancer cell lines, the latter more likely attributable to enhanced daily bowel movements and increased intestinal fecal content of lactic acid bacteria, which would finally contribute to lower the risk of malignancies, especially colorectal cancer.

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PMID: 

Respir Physiol Neurobiol. 2019 Aug 9:103265. Epub 2019 Aug 9. PMID: 31404684

Abstract Title: 

Combination of ascorbic acid and calcitriol attenuates chronic asthma disease by reductions in oxidative stress and inflammation.

Abstract: 

Airway inflammation and oxidative stress are the two major characteristics of asthma pathogenesis. Therefore, this study evaluated the protective effects of ascorbic acid in combination with calcitriol on the oxidative damages and inflammation in asthma model. All animals, except in the control group, were sensitized and challenged with ovalbumin. One day after the last challenge, samples of bronchoalveolar lavage fluid was collected for the assessment of total white blood cell counts and differential count of white blood cell and plasma was used for the measurement of pro-oxidant/antioxidant balance level. Lung tissue samples were also stored for examining peribronchial inflammatory cell infiltration, phosphorylated nuclear factor-kappa B expression and measurement of malondialdehyde level. Induction of asthma caused significant increases in total white blood cell counts, percentage of neutrophils and eosinophils and a decrease in the percentage of lymphocytes. Moreover, asthma resulted in significant increases of peribronchial inflammatory cell infiltration, phosphorylated nuclear factor-kappa B expression and malondialdehyde level. However, no significant changes were observed in pro-oxidant/antioxidant balance level with the induction of asthma. Co-administration of low doses of ascorbic acid and calcitriol returned all to the levels measured before sensitization and challenge. Combination of low doses of ascorbic acid with calcitriol improves mouse asthma model by a possible additive effects through the decrease of oxidative stress and inflammation.

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PMID: 

Electromagn Biol Med. 2008 ;27(2):205-9. PMID: 18568938

Abstract Title: 

Some ocular symptoms experienced by users of mobile phones.

Abstract: 

This survey study was conducted, using a questionnaire, on 229 university students (181 women, 48 men) in Kocaeli, Turkey. Six ocular symptoms experienced during use of mobile phones were studied by means of the chi-square test with Yates correction. The studied symptoms were blurring of vision, redness of the eyes, vision disturbance, secretion of the eyes, inflammation in the eyes, and lachrymation of the eyes. A significant increase in blurring of vision (p2 years compared to users of mobile phone possession

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PMID: 

Redox Biol. 2019 Aug 2 ;26:101290. Epub 2019 Aug 2. PMID: 31412312

Abstract Title: 

Redox modifications of cysteine-containing proteins, cell cycle arrest and translation inhibition: Involvement in vitamin C-induced breast cancer cell death.

Abstract: 

Vitamin C (VitC) possesses pro-oxidant properties at high pharmacologic concentrations which favor repurposing VitC as an anti-cancer therapeutic agent. However, redox-based anticancer properties of VitC are yet partially understood. We examined the difference between the reduced and oxidized forms of VitC, ascorbic acid (AA) and dehydroascorbic acid (DHA), in terms of cytotoxicity and redox mechanisms toward breast cancer cells. Our data showed that AA displayed higher cytotoxicity towards triple-negative breast cancer (TNBC) cell lines in vitro than DHA. AA exhibited a similar cytotoxicity on non-TNBC cells, while only a minor detrimental effect on noncancerous cells. Using MDA-MB-231, a representative TNBC cell line, we observed that AA- and DHA-induced cytotoxicity were linked to cellular redox-state alterations. Hydrogen peroxide (HO) accumulation in the extracellular medium and in different intracellular compartments, and to a lesser degree, intracellular glutathione oxidation, played a key role in AA-induced cytotoxicity. In contrast, DHA affected glutathione oxidation and had less cytotoxicity. A"redoxome"approach revealed that AA treatment altered the redox state of key antioxidants and a number of cysteine-containing proteins including many nucleic acid binding proteins and proteins involved in RNA and DNA metabolisms and in energetic processes. We showed that cell cycle arrest and translation inhibition were associated with AA-induced cytotoxicity. Finally, bioinformatics analysis and biological experiments identified that peroxiredoxin 1 (PRDX1) expression levels correlated with AA differential cytotoxicity in breast cancer cells, suggesting a potential predictive value of PRDX1. This study provides insight into the redox-based mechanisms of VitC anticancer activity, indicating that pharmacologic doses of VitC and VitC-based rational drug combinations could be novel therapeutic opportunities for triple-negative breast cancer.

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PMID: 

Eur J Cancer Prev. 2003 Aug ;12(4):301-7. PMID: 12883383

Abstract Title: 

Follow-up of radio and telegraph operators with exposure to electromagnetic fields and risk of breast cancer.

Abstract: 

It is still unclear whether exposure to electromagnetic fields (EMF) is associated with breast cancer. To further investigate the issue, we followed-up a cohort of Norwegian female radio and telegraph operators in the period 1 January 1961 to 31 May 2002, with 99 breast cancer cases. The standardized incidence ratio (SIR) for breast cancer was 1.30 (95% confidence intervals (CI) 1.05-1.58), compared with the total Norwegian female population. In a subsequent nested case-control study, exposure to radio frequency (405 kHz-25 MHz) and extremely low-frequency (50 Hz) fields due to stay in the radio room during day and night was cumulated by years of employment and workload according to ship type. The exposure was assessed in two age groups (

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