PMID: 

Molecules. 2015 Jul 9 ;20(7):12500-11. Epub 2015 Jul 9. PMID: 26184136

Abstract Title: 

Constituents of Psoralea corylifolia Fruits and Their Effects on Methicillin-Resistant Staphylococcus aureus.

Abstract: 

Two new flavonoids, bakuisoflavone (1) and bakuflavanone (2), together with 15 known compounds, were isolated from the fruits of Psoralea corylifolia, and their structures were characterized by spectroscopic data. The effects of the isolated compounds on methicillin-resistant Staphylococcus aureus were also examined. We found that two compounds, isobavachalcone (10) and bakuchiol (12), showed noticeable antibacterial effects on the MRSA strains examined. Quantitation of the major constituents, including anti-MRSA constituents, was then performed. The results showed individual contents of 1.26%-16.49% (w/w) among the examined compounds in the ethyl acetate extract from P. corylifolia fruits.

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PMID: 

Oxid Med Cell Longev. 2019 ;2019:4310319. Epub 2019 Dec 26. PMID: 31976027

Abstract Title: 

L. Seed Extract Attenuates Methylglyoxal-Induced Insulin Resistance by Inhibition of Advanced Glycation End Product Formation.

Abstract: 

Accumulation of advanced glycation end products (AGEs) in the body has been implicated in the pathogenesis of metabolic conditions, such as diabetes mellitus. Methylglyoxal (MGO), a major precursor of AGEs, has been reported to induce insulin resistance in bothandstudies.seeds (PCS) have been used as a traditional medicine for several diseases, but their potential application in treating insulin resistance has not yet been evaluated. This study is aimed at investigating whether PCS extract could attenuate insulin resistance induced by MGO. Male C57BL/6N mice (6 weeks old) were administered 1% MGO in their drinking water for 18 weeks, and the PCS extract (200 or 500 mg/kg) was orally administered daily from the first day of the MGO administration. We observed that both 200 and 500 mg/kg PCS extract treatment significantly improved glucose tolerance and insulin sensitivity and markedly restored p-Akt and p-IRS1/2 expression in the livers of the MGO-administered mice. Additionally, the PCS extract significantly increased the phosphorylation of Akt and IRS-1/2 and glucose uptake in MGO-treated HepG2 cells. Further studies showed that the PCS extract inhibited MGO-induced AGE formation in the HepG2 cells and in the sera of MGO-administered mice. PCS extract also increased the expression of glyoxalase 1 (GLO1) in the liver tissue of MGO-administered mice. The PCS extract significantly decreased the phosphorylation of ERK, p38, and NF-B and suppressed the mRNA expression of proinflammatory molecules including TNF-and IL-1and iNOS in MGO-administered mice. Additionally, we demonstrated that the PCS extract attenuated oxidative stress, as evidenced by the reduced ROS production in the MGO-treated cells and the enhanced expression of antioxidant enzymes in the liver of MGO-administered mice. Thus, PCS extract ameliorated the MGO-induced insulin resistance in HepG2 cells and in mice by reducing oxidative stress via the inhibition of AGE formation. These findings suggest the potential of PCS extract as a candidate for the prevention and treatment of insulin resistance.

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PMID: 

Br J Nutr. 2012 Apr ;107(7):1037-47. Epub 2011 Aug 3. PMID: 21810288

Abstract Title: 

Effect of 6 weeks' consumption ofβ-glucan-rich oat products on cholesterol levels in mildly hypercholesterolaemic overweight adults.

Abstract: 

Several regulatory bodies have approved a health claim on the cholesterol-lowering effects of oatβ-glucan at levels of 3·0 g/d. The present study aimed to test whether 1·5 g/d β-glucan provided as ready-to-eat oat flakes was as effective in lowering cholesterol as 3·0 g/d from oats porridge. A 6-week randomised controlled trial was conducted in eighty-seven mildly hypercholesterolaemic ( ≥5 mmol/l and

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PMID: 

Nutr J. 2011 Nov 25 ;10:130. Epub 2011 Nov 25. PMID: 22118569

Abstract Title: 

Bioactive oatβ-glucan reduces LDL cholesterol in Caucasians and non-Caucasians.

Abstract: 

BACKGROUND: There is increasing global acceptance that viscous soluble fibers lower serum LDL cholesterol (LDL-C), but most evidence for this comes from studies in Caucasians. To see if oatβ-glucan lowers LDL-C in Caucasians and non-Caucasians we conducted a post-hoc analysis of the results of a randomized, controlled, double-blind, multi-center clinical trial whose primary aim was to determine if molecular-weight (MW) influenced the LDL-C-lowering effect of oat β-glucan.RESULTS: Caucasian and non-Caucasian subjects with LDL-C-C≥ 3.0 and ≤ 5.0 mmol/L (n = 786 screened, n = 400 ineligible, n = 19 refused, n = 367 randomized, n = 345 completed, n = 1 excluded for missing ethnicity) were randomly assigned to consume cereal containing wheat-fiber (Control, n = 74:13 Caucasian:non-Caucasian) or 3 g high-MW (3H, 2,250,000 g/mol, n = 67:19), 4 g medium-MW (4 M, 850,000 g/mol, n = 50:17), 3 g medium-MW (3M, 530,000 g/mol, n = 54:9) or 4 g low-MW (4 L, 210,000 g/mol, n = 51:12) oat β-glucan daily for 4 weeks. LDL-C after 4 weeks was influenced by baseline LDL-C (p

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PMID: 

Br J Nutr. 2013 Dec ;110(12):2173-9. Epub 2013 Jun 7. PMID: 23742725

Abstract Title: 

Molecular weight of barleyβ-glucan influences energy expenditure, gastric emptying and glycaemic response in human subjects.

Abstract: 

Barleyβ-glucan (BG) has been shown to reduce glycaemic response (GR) in some studies. It is hypothesised that this reduction may be a function of its physical properties that delay gastric emptying (GE). The effect of these changes in GR and GE on diet-induced thermogenesis (DIT) is not known. The aim ofthe present study was to assess the effect of BG of different molecular weights and purities on GR, GE and DIT in healthy subjects. This was a randomised, single-blind, repeated-measures design where fifteen healthy subjects were tested on three occasions following an overnight fast. Following thebaseline measurements, the volunteers were fed a soup containing high-molecular-weight BG (HBG), a soup containing low-molecular-weight BG (LBG) or a control soup with no BG (CHO). Following the consumption of the breakfast, GR was measured using finger-prick blood samples, GE was determined using the 13C-octanoic acid breath test and DIT was measured using indirect calorimetry. There was a difference in GR AUC between the soups after 60 min but not after 120 min. The CHO and LBG meals had a greater GR than the HBG meal. There were differences in all GE time points, with the HBG meal having the slowest GE time. There was a correlation between the GR and the initial GE times. There were differences in total DIT between the three test meals with the HBG meal having the lowest DIT. The present study indicates that HBG has the ability to delay GE due to increased viscosity, resulting in a decreased GR and DIT.

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PMID: 

J Diet Suppl. 2013 Sep ;10(3):171-83. Epub 2013 Aug 9. PMID: 23927572

Abstract Title: 

Baker's yeast beta glucan supplementation increases salivary IgA and decreases cold/flu symptomatic days after intense exercise.

Abstract: 

Strenuous exercise, such as running a marathon, is known to suppress mucosal immunity for up to 24 hr, which can increase the risk of developing an upper respiratory tract infection (URTI) and reduced performance capacity (Allgrove JE, Geneen L, Latif S, Gleeson M. Influence of a fed or fasted state on the s-IgA response to prolonged cycling in active men and women. Int J Sport Nutr Exerc Metab. 2009;19(3):209-221; Barrett B, Locken K, Maberry R, Schwamman J, Brown R, Bobula J, Stauffacher EA. The Wisconsin Upper Respiratory Symptom Survey (WURSS): a new research instrument for assessing the common cold. J Fam Pract. 2002;51(3):265; Carpenter KC, Breslin WL, Davidson T, Adams A, McFarlin BK. Baker's yeast beta glucan supplementation increases monocytes and cytokines post-exercise: implications for infection risk? Br J Nutr. 2012;1-9). While many dietary interventions have been used to combat postexercise immune suppression, most have been ineffective. The key purpose of this study was to determine if baker's yeastβ-glucan (BG) could positively affect the immune system of individuals undergoing intense exercise stress using two experiments. In the first (E1; N = 182 men and women), BG was compared to placebo supplementation for the incidence of URTI symptoms for 28 days postmarathon. In the second (E2; N = 60 men and women) changes in salivary immunoglobulin A (IgA) were evaluated after 50-min of strenuous cycling when participants had been supplemented for 10 days with either BG (250 mg/day) or placebo (rice flour). For E1, subjects reported URTI symptoms using a daily health log. For E2, saliva was collected prior to, immediately, and 2-hr postexercise using a salivette. Data for E1 and E2 were analyzed using separate analyses of variance (ANOVAs) with repeated measures (p

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PMID: 

Phytochemistry. 2017 Nov ;143:186-193. Epub 2017 Aug 18. PMID: 28825980

Abstract Title: 

Isoflavones and anti-inflammatory constituents from the fruits of Psoralea corylifolia.

Abstract: 

The fruits of Psoralea corylifolia, known as Psoraleae Fructus (Buguzhi in Chinese), are traditionally used for the treatment of spermatorrhea, nephritis, asthma, pollakiuria, and various inflammatory diseases. Three previously undescribed isoflavone derivatives, 7-O-methylcorylifol A, 7-O-isoprenylcorylifol A, and 7-O-isoprenylneobavaisoflavone, have been isolated from the fruits of P. corylifolia, together with 9 known compounds. The structures of these compounds were determined through spectroscopic and MS analyses. Among the isolated compounds, 7-O-methylcorylifol A and psoralen exhibited potent inhibition (ICvalues ≤ 10.89 μM) of superoxide anion generation by human neutrophils in response to N-formyl-L-methionyl-L-leucyl-L-phenylalanine/cytochalasin B (fMLP/CB). 7-O-Isoprenylcorylifol A, 7-O-isoprenylneobavaisoflavone, and 12,13-dihydro-12,13-epoxybakuchiol inhibited fMLP/CB-induced elastase release with ICvalues ≤ 14.30 μM. In addition, 7-O-isoprenylcorylifol A, bakuchiol, 12,13-dihydro-12,13-epoxybakuchiol, and psoralidin showed potent inhibition with ICvalues ≤ 36.65 μM, against lipopolysaccharide (LPS)-induced nitric oxide (NO) generation.

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PMID: 

J Med Assoc Thai. 2013 Dec ;96 Suppl 5:S25-32. PMID: 24851570

Abstract Title: 

Effect of oat consumption on lipid profiles in hypercholesterolemic adults.

Abstract: 

BACKGROUND: Hypercholesterolemia is a strong risk factor in cardiovascular disease. Oat (Avena sativa L.) beta-glucan, the soluble fiber in oat, has been known to reduce blood cholesterol levels considerably. However, the effect of oat soluble fiber in the Thai population is unknown.OBJECTIVE: To determine the effect of oat consumption on serum lipid profiles in Thai hypercholesterolemic adults.MATERIAL AND METHOD: The present study was a randomized, crossover design. Twenty-four hypercholesterolemic adults, male and female aged 30-60 years, were randomly assigned into two groups of twelve each. Group 1 consumed 70 g (3 g beta-glucan) of oatmeal daily through the first 4-week intervention, and then switched to 70 g rice porridge (control product) daily for the next 4-week intervention. Group 2 consumed rice porridge first and then oatmeal. Before and after each intervention period, lipid profiles including total cholesterol (TC), triglycerides (TG), high density lipoprotein cholesterol (HDL-C), and low density lipoprotein cholesterol (LDL-C) of all subjects were measured.RESULTS: Following daily oat consumption, total cholesterol and LDL-cholesterol levels were significantly lower than baseline levels and lower than the levels observed with rice consumption. Oat consumption reduced total cholesterol by 5% and LDL-cholesterol by 10% from baseline levels. In addition, mean and percent changes were significantly different from the levels after consuming rice porridge (p

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PMID: 

Fitoterapia. 2019 Nov ;139:104373. Epub 2019 Oct 16. PMID: 31629053

Abstract Title: 

Anti-diabetic compounds from the seeds of Psoralea corylifolia.

Abstract: 

A new aurone named (2Z)-2-[(4'-hydroxyphenyl) methylene]-6-hydroxy-7-prenyl-3(2H)-benzofurane (1), two new flavonoids named (2S)-7-methoxy-6-(2-hydroxy-3-methylbut-3-en-1-yl)-2-(4-hydroxyphenyl)chroman-4-one (2), (2S)-4'-hydroxyl-7-hydroxymethylene-6-(2″,3″-epoxy-3″-methylbutyl)flavanone (3), and a new coumestan named bavacoumestan E (4), together with eleven known compounds (5-15), were isolated from the seeds of Psoralea corylifolia. The chemical structures were elucidated by spectroscopic and physico-chemical analyses. All isolates were evaluated for in vitro inhibitory activity against DGAT, PTP1B and α-glucosidase. Compounds 1, 2 and 3 showed potential inhibitory activities on DGAT1 with ICvalues of 35.2 ± 1.3, 51.3 ± 1.1 and 43.4 ± 0.7 μM, respectively. Compounds 6 and 8 displayed the significant inhibitory activities on α-glucosidase with ICvalue of 28.0 and 23.0 μM, respectively.

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PMID: 

Molecules. 2016 Aug 17 ;21(8). Epub 2016 Aug 17. PMID: 27548120

Abstract Title: 

Quantitative Analysis of Psoralea corylifolia Linne and its Neuroprotective and Anti-Neuroinflammatory Effects in HT22 Hippocampal Cells and BV-2 Microglia.

Abstract: 

The seeds of Psoralea corylifolia L. (P. corylifolia), also known as"Bo-Gol-Zhee"in Korea, are used in a traditional herbal medicine for treating various skin diseases. In the present study, we performed quantitative analyses of the seven standard components of P. corylifolia: psoralen, angelicin, neobavaisoflavone, psoralidin, isobavachalcone, bavachinin, and bakuchiol, using high-performance liquid chromatography. We also investigated the neuroprotective and anti-neuroinflammation effects of P. corylifolia and its standard components in the hippocampal cell line HT22 and microglia cell line BV-2. A 70% ethanol extract of P. corylifolia was prepared and the seven standard components were separated using C-18 analytical columns by gradient solvents with acetonitrile and water, and ultraviolet detection at 215, 225 and 275 nm. The analytical method showed high linearity, with a correlation coefficient of≥0.9999. The amounts of the standard components ranged from 0.74 to 11.71 mg/g. Among the components, bakuchiol (11.71 mg/g) was the most potent phytochemical component of P. corylifolia. Furthermore, we analyzed the inhibitory effects of the components from P. corylifolia to determine the bioactive compound needed to regulate neuronal cell changes. Angelicin, isobavachalcone, and bakuchiol suppressed lipopolysaccharide (LPS)-stimulated nitric oxide production in LPS-treated BV-2 microglia more significantly than did the other components. In HT22 hippocampal cells, neobavaisoflavone and bakuchiol had more potent inhibitory activity against hydrogen peroxide-induced cell death. Taken together of the quantification and efficacy analyses, bakuchiol appeared to be the most potent bioactive phytochemical component of P. corylifolia for the potential treatment of neurodegenerative diseases.

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