The History of CBD

CBD is new to many and with the explosion of the CBD marketplace many are benefiting from the healing properties of the cannabinoid. While it might seem new, CBD has been used for centuries.

The History of CBD

The first known use of marijuana for medicinal purposes was in 2737 B.C. Emperor Shen Neng of China prescribed marijuana for many of the same aliments the plant helps with today- gout, rheumatism, and pain relief. News of this treatment spread throughout Asia and the use of marijuana as medicine grew.

Reports claim that Christopher Columbus brought cannabis on his trip in 1492. Later in 1619, law in Jamestown required citizens to grow cannabis. George Washington even grew cannabis for fibers at his Mount Vernon estate. Marijuana and hemp were both commonly used in North America for medicinal purposes by the nineteenth century. Dr. William O’Shaughnessy, an Irish doctor who helped popularize medical marijuana use in England and Europe conducted a study examining the medical properties of marijuana.

In 1833, O’Shaughnessy began working for the East India Company and moved to Calcutta to work as a surgeon. There, he learned about marijuana from indigenous people and developed recipes for aliments using cannabis. O’Shaughnessy conducted many studies on patients using cannabis and treated convulsions and pain. His research also revealed the different compounds in the plant which we now know as cannabinoids.

After O’Shaughnessy brought marijuana into Western medicine, it became a popular medical treatment and alternative to morphine. The Marijuana Tax Act of 1937 was passed by Congress, which made “the individual possession and sale of marijuana” illegal. However, the ruling did not outlaw medicinal marijuana but required those who “bought, sold, imported, distributed, cultivated or prescribed it as medicine” to pay a tax. The passing of this act in 1937 limited the studies and further development of medical marijuana.

The plant was still studied by researchers to better understand its properties. Using O’Shaughnessy’s research, British chemist Robert S. Cahn discovered individual cannabinoids in 1940.

In 1942, CBD itself was discovered by Roger Adams, an American chemist who successfully isolated the compound. His research also led to the discovery of THC.

More studies into marijuana and CBD were conducted, which led to the CBD boom of today. In 1963, Dr. Raphael Mechoulam discovered the individual effects of cannabinoids. In the 1980s, Mechoulam discovered CBD can aid in the treatment of seizures, a common use today.

Since many states have made medical marijuana legal and CBD was made federally legal in 2018, the use of cannabis and cannabinoids as medicine has become de-stigmatized. Experts expect the CBD market to be worth an estimated $22 billion by 2022.

The anti-proliferative and anti-invasive effect of leaf extracts of blueberry plants.

PMID: 

Antioxidants (Basel). 2020 Jan 4 ;9(1). Epub 2020 Jan 4. PMID: 31948009

Abstract Title: 

The Anti-Proliferative and Anti-Invasive Effect of Leaf Extracts of Blueberry Plants Treated with Methyl Jasmonate on Human Gastric Cancer In Vitro Is Related to Their Antioxidant Properties.

Abstract: 

Gastric cancer is the third main cause of cancerous tumors in humans in Chile. It is well-accepted that a diet rich in antioxidant plants could help in fighting cancer. Blueberry is a fruit crop with a high content of antioxidants. Methyl jasmonate (MeJA) is a phytohormone involved in plant defenses under stress conditions. The exogenous application of MeJA can improve the antioxidant properties in plants. We studied in vitro and in vivo anticancer action on human gastric cancer (cell line AGS) and the antioxidant properties of extracts from blueberry plants untreated and treated with MeJA. The results demonstrated that leaf extracts displayed a higher inhibition of cancer cell viability as well as greater antioxidant properties compared to fruit extracts. Besides, MeJA applications to plants improved the antioxidant properties of leaf extracts (mainly anthocyanins), increasing their inhibition levels on cell viability and migration. It is noteworthy that leaf extract from MeJA-treated plants significantly decreased cancer cell migration and expression of gastric cancer-related proteins, mainly related to the mitogen-activating protein kinase (MAPK) pathway. Interestingly, in all cases the anticancer and antioxidant properties of leaf extracts were strongly related. Despite highlighted outcomes, in vivo results did not indicate significant differences incolonization nor inflammation levels in Mongolian gerbils unfed and fed with blueberry leaf extract. Our findings demonstrated that MeJA increased antioxidant compounds, mainly anthocyanins, and decreased the viability and migration capacity of AGS cells. In addition, leaf extracts from MeJA-treated plants were also able to decrease the expression of gastric cancer-related proteins. Our outcomes also revealed that the anthocyanin-rich fraction of blueberry leaf extracts showed higher in vitro antiproliferative and anti-invasive effects than the crude leaf extracts. However, it is still uncertain whether the leaf extracts rich in anthocyanins of blueberry plants are capable of exerting a chemopreventive or chemoprotective effect against gastric cancer on an in vivo model.

read more

Selenium anticancer properties and impact on cellular redox status.

PMID: 

Antioxidants (Basel). 2020 Jan 17 ;9(1). Epub 2020 Jan 17. PMID: 31963404

Abstract Title: 

Selenium Anticancer Properties and Impact on Cellular Redox Status.

Abstract: 

(1) Background: In this review, we provide information published in recent years on the chemical forms, main biological functions and especially on antioxidant and prooxidant activities of selenium. The main focus is put on the impact of selenoproteins on maintaining cellular redox balance and anticancerogenic function. Moreover, we summarize data on chemotherapeutic application of redox active selenium compounds. (2) Methods: In the first section, main aspects of metabolism and redox activity of selenium compounds is reviewed. The second outlines multiple biological functions, asserted when selenium is incorporated into the structure of selenoproteins. The final section focuses on anticancer activity of selenium and chemotherapeutic application of redox active selenium compounds as well. (3) Results: optimal dietary level of selenium ensures its proper antioxidant and anticancer activity. We pay special attention to antioxidant activities of selenium compounds, especially selenoproteins, and their importance in antioxidant defence. It is worth noting, that data on selenium anticancer properties is still contraversive. Moreover, selenium compounds as chemotherapeutic agents usually are used at supranutritional doses. (4) Conclusions: Selenium play a vital role for many organism systems due to its incorporation into selenoproteins structure. Selenium possesses antioxidant activity at optimal doses, while at supranutritional doses, it displays prooxidant activity. Redox active selenium compounds can be used for cancer treatment; recently special attention is put to selenium containing nanoparticles.

read more

Inflammation in post-traumatic stress disorder: a review of potential correlates of PTSD with a neurological perspective.

PMID: 

Antioxidants (Basel). 2020 Jan 26 ;9(2). Epub 2020 Jan 26. PMID: 31991875

Abstract Title: 

Inflammation in Post-Traumatic Stress Disorder (PTSD): A Review of Potential Correlates of PTSD with a Neurological Perspective.

Abstract: 

Post-traumatic stress disorder (PTSD) is a chronic condition characterized by symptoms of physiological and psychosocial burden. While growing research demonstrated signs of inflammation in PTSD, specific biomarkers that may be representative of PTSD such as the detailed neural correlates underlying the inflammatory responses in relation to trauma exposure are seldom discussed. Here, we review recent studies that explored alterations in key inflammatory markers in PTSD, as well as neuroimaging-based studies that further investigated signs of inflammation within the brain in PTSD, as to provide a comprehensive summary of recent literature with a neurological perspective. A search was conducted on studies published from 2009 through 2019 in PubMed and Web of Science. Fifty original articles were selected. Major findings included elevated levels of serum proinflammatory cytokines in individuals with PTSD across various trauma types, as compared with those without PTSD. Furthermore, neuroimaging-based studies demonstrated that altered inflammatory markers are associated with structural and functional alterations in brain regions that are responsible for the regulation of stress and emotion, including the amygdala, hippocampus, and frontal cortex. Future studies that utilize both central and peripheral inflammatory markers are warranted to elucidate the underlying neurological pathway of the pathophysiology of PTSD.

read more

Raw milk, but not heat-treated milk, displays strong viral inhibition of bovine rotavirus and coronavirus.

PMID: 

J Food Prot. 1987 Oct ;50(10):862-866. PMID: 30978786

Abstract Title: 

Antiviral Substances in Raw Bovine Milk Active Against Bovine Rotavirus and Coronavirus.

Abstract: 

After experimental contamination of bovine raw and heat-treated milks with bovine rotavirus and coronavirus strains, we observed a strong viral inhibition only with raw milks, from which virus recovery was 5× 10%. Between 30% and 80% of the virus was recovered from the heat-treated milks, depending on the level of inoculation. The antiviral substance is heat-labile (destroyed within 30 min at 100°C), precipitated by ammonium sulfate and filtrable (0.45 μm Millipore membrane). It also has neutralizing activity on tissue culture.

read more

Flavonoids display significant antiviral activity, including against coronavirus.

PMID: 

Microbiologica. 1990 Jul ;13(3):207-13. PMID: 2125682

Abstract Title: 

Effects of propolis flavonoids on virus infectivity and replication.

Abstract: 

The effect of five propolis flavonoids on the infectivity and replication of some herpesvirus, adenovirus, coronavirus and rotavirus strains has been studied. Experiments were performed in vitro in cell cultures using the viral plaque reduction technique. The cytotoxicity of flavonoids, including chrysine, kaempferol, acacetin, galangin and quercetin, was evaluated on uninfected monolayers to determine their effect on cell growth and viability. Chrysine and kaempferol caused a concentration-dependent reduction of intracellular replication of herpes-virus strains when monolayers were infected and subsequently cultured in a drug-containing medium. However, virus infectivity was not significantly affected. Acacetin and galangin had no effect on either the infectivity or replication of any of the viruses studied. Quercetin reduced infectivity and intracellular replication, but only at the highest concentrations tested.

read more

12 British Columbian medicinal plants display antiviral activity, including against coronaviridiae.

PMID: 

J Ethnopharmacol. 1995 Dec 1 ;49(2):101-10. PMID: 8847882

Abstract Title: 

Antiviral screening of British Columbian medicinal plants.

Abstract: 

One hundred methanolic plant extracts were screened for antiviral activity against seven viruses. Twelve extracts were found to have antiviral activity at the non-cytotoxic concentrations tested. The extracts of Rosa nutkana and Amelanchier alnifolia, both members of the Rosaceae, were very active against an enteric coronavirus. A root extract of another member of the Rosaceae, Potentilla arguta, completely inhibited respiratory syncytial virus. A Sambucus racemosa branch tip extract was also very active against respiratory syncytial virus while the inner bark extract of Oplopanax horridus partially inhibited this virus. An extract of Ipomopsis aggregata demonstrated very good activity against parainfluenza virus type 3. A Lomatium dissectum root extract completely inhibited the cytopathic effects of rotavirus. In addition to these, extracts prepared from the following plants exhibited antiviral activity against herpesvirus type 1: Cardamine angulata, Conocephalum conicum, Lysichiton americanum, Polypodium glycyrrhiza and Verbascum thapsus.

read more

Immune activity against coronavirus infections are improved by omega-6 polyunsaturated fatty acids in turkeys.

PMID: 

Br Poult Sci. 1997 Sep ;38(4):342-8. PMID: 9347140

Abstract Title: 

Effect of dietary fatty acids on humoral immune response of turkeys.

Abstract: 

1. This study examined the effect of increasing amounts of dietary polyunsaturated fatty acids on the fatty acid composition in serum and antibody production following a standard vaccination programme in growing turkeys. Turkey poults were fed on 5 diets containing 75g/kg added fat made up of different proportions of palm and soyabean oils, and were vaccinated against Newcastle disease, infectious bronchitis and necrotic enteritis according to a standard vaccination programme. Blood samples were taken before and one week after each vaccination. 2. Fatty acid composition in serum reflected the composition of the diets although arachidonic acid concentration was not changed by dietary fatty acid content. Growth, erythrocyte and leukocyte parameters were not affected by the respective diets. 3. Specific antibody production was related quadratically to serum linoleic and total n-6 polyunsaturated fatty acid concentrations. No correlation was found with linolenic or arachidonic acids. 4. It is concluded that dietary fatty acid composition can augment the specific anti-vaccine immune response in turkey poults.

read more

Theaflavins from black tea exhibit antiviral activity against bovine rotavirus and bovine coronavirus infections.

PMID: 

Vet Microbiol. 1998 Oct ;63(2-4):147-57. PMID: 9850995

Abstract Title: 

An in vitro study of theaflavins extracted from black tea to neutralize bovine rotavirus and bovine coronavirus infections.

Abstract: 

Crude theaflavin was extracted from black tea and then fractionated by HPLC into five components (initial peaks (IP), TF1, TF2A, TF2B, and TF3). The crude extract and the various fractions of theaflavin were collected and tested, individually and in combination, for antirotaviral activity. The mean effective concentration (EC50) was calculated and compared. Activity varied from the most active being the uncharacterized theaflavin-like initial peaks (IP) with an EC50 of 0.125 microgram/ml to the least active being theaflavin-3 monogallate (TF2A) with an EC50 of 251.39 micrograms/ ml. The combination of TF1 + TF2A + TF2B + TF3 was more active than the sum of the activities of these four fractions individually, indicating synergism among the peaks. Only the crude extract was assayed for activity against coronavirus; the EC50 was 34.7 micrograms/ml.

read more

Curcumin protects human trophoblast HTR8/SVneo cells from H2O2-induced oxidative stress.

PMID: 

Antioxidants (Basel). 2020 Feb 1 ;9(2). Epub 2020 Feb 1. PMID: 32024207

Abstract Title: 

Curcumin Protects Human Trophoblast HTR8/SVneo Cells from HO-Induced Oxidative Stress by Activating Nrf2 Signaling Pathway.

Abstract: 

Pregnancy complications are associated with oxidative stress induced by accumulation of trophoblastic ROS in the placenta. We employed the human trophoblast HTR8/SVneo cell line to determine the effect of curcumin pre-treatment on HO-induced oxidative damage in HTR8/Sveo cells. Cells were pretreated with 2.5 or 5μM curcumin for 24 h, and then incubated with 400 μM HOfor another 24 h. The results showed that HOdecreased the cell viability and induced excessive accumulation of reactive oxygen species (ROS) in HTR8/Sveo cells. Curcumin pre-treatment effectively protected HTR8/SVneo cells against oxidative stress-induced apoptosis via increasing Bcl-2/Bax ratio and decreasing the protein expression level of cleaved-caspase 3. Moreover, curcumin pre-treatment alleviated the excessive oxidative stress by enhancing the activity of antioxidative enzymes. The antioxidant effect of curcumin was achieved by activating Nrf2 and its downstream antioxidant proteins. In addition, knockdown of Nrf2 by Nrf2-siRNA transfection abolished the protective effects of curcumin on HTR8/SVneo cells against oxidative damage. Taken together, our results show that curcumin could protect HTR8/SVneo cells from HO-induced oxidative stress by activating Nrf2 signaling pathway.

read more

Design a site like this with WordPress.com
Get started