PMID: 

Adv Mind Body Med. 2018 Summer;32(3):18-22. PMID: 31370033

Abstract Title: 

Effects of Integrated Yoga Intervention on Psychopathologies and Sleep Quality Among Professional Caregivers of Older Adults With Alzheimer's Disease: A Controlled Pilot Study.

Abstract: 

Context: Providing care to patients suffering from chronic neurological problems is a stressful job. While providing care to the patients, professional caregivers experience various kinds of physical and mental challenges that affect their mental health and sleep. Yoga is a form of mind-body medicine shown to be an effective intervention in improving physical and mental health.Objective: To examine the effects of an integrated yoga (IY) intervention on anxiety, depression, stress, and sleep quality among professional caregivers of older adults with Alzheimer's disease.Setting: This study was conducted in an Alzheimer care institution located in Bangalore City in southern India.Participants: Participants were professional female caregivers of older adults with Alzheimer's disease. Participant age range was between 20 and 50 y (mean, 34± 8.4 y). A total of 30 participants were enrolled in the study. Seventeen participants followed IY intervention and 13 were considered in a wait-list group.Intervention: Participants in the IY group received a structured IY intervention comprising yoga asanas, pranayama, meditation, and relaxation techniques, 1 h/d, 6 d/wk, for 1 mo. Participants in the wait-list control group followed their daily activities.Outcome Measures: Blood pressure, heart rate, anxiety, depression, stress, and sleep quality were assessed at baseline after 1 mo for both the groups. Data were analyzed with an appropriate statistical test using SPSS Version 16 software (IBM, Armonk, NY, USA).Results: The IY group showed significant improvement in heart rate, blood pressure, stress, depression, anxiety, and sleep quality after 1 mo compared with baseline. In contrast to the IY group, the wait-listed control group showed significant increase in anxiety, depression, and stress and significant decrease in sleep quality after 1 mo compared with baseline.Conclusions: The present study showed the potential use of IY intervention in reducing stress, anxiety, and depression. The study also suggests that IY improves sleep quality among professional caregivers. However, further studies using a randomized controlled trial method with a larger sample size and for a longer duration should be conducted to confirm the present findings.

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PMID: 

Adv Mind Body Med. 2018 Fall;32(4):4-8. PMID: 31370034

Abstract Title: 

How Does Yoga Work on Pain Dimensions? An Integrated Perspective in 2 Individuals With Fibromyalgia.

Abstract: 

Context: The effectiveness of yoga has been studied in fibromyalgia (FM) using improvement in its 5 key dimensions-pain, quality of life, sleep, depression, and disability-as outcome measures. Studies have demonstrated an improvement in the psychosocial dimensions of pain after yoga practice, but these findings failed to reach statistical significance. Although studies have shown the efficacy of yoga in the modulation of pain, no study has yet investigated how it acts on each dimension of pain.Objective: The study intended to investigate the dimensions of pain-sensory, evaluative, and/or affective-and which psychological comorbidities-anxiety and/or depression-that Hatha yoga affects in individuals with FM.Design: The research team performed 2 case studies.Setting: The study occurred at the GIFT Institute of Integrative Medicine (Pisa, Italy).Participants: Participants were 2 patients at the institute who had FM.Intervention: At baseline (T0), participants were prescribed 8 mo of pharmacological treatment. At 2 mo after baseline (T1), they participated in an 8-h, mind-body, psychoeducational course (PEC) for self-management of chronic pain. Each participant was contacted by phone every week for 2 mo after the PEC (ie, until 4 mo from baseline (T2). For the next 2 mo, participants had no contact with a health care practitioner, to sustain a deeper PEC program. Participants then took a 2-mo Hatha yoga program from months 6 (T3) to 8 (T4).Outcome Measures: Sensorial, affective, and evaluative dimensions of pain were investigated using the Italian Pain Questionnaire, and depression and anxiety were investigated using the Hospital Anxiety Depression at T0, T1, T2, T3, and T4. The IPQ was administered weekly, before and after each yoga session.Results: Hatha yoga proved to be an effective means of relieving pain in FM. In particular, a measurable improvement in scores occurred for the affective dimension of pain after only 4 yoga sessions; this effect remained stable throughout the remainder of the program.Conclusions: Monitoring the affective dimension of pain should be included in an integrated approach to pain, and Hatha yoga may be beneficial in the pain management of FM participants.

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PMID: 

Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2010 Jun ;18(3):629-33. PMID: 20561416

Abstract Title: 

[Apoptosis-inducing effect of quercetin and kaempferol on human HL-60 cells and its mechanism].

Abstract: 

The purpose of this study was to explore the anti-leukemia effect of quercetin and kaempferol and its mechanism. The HL-60 cells were used as the leukemia models. The inhibitory effects of quercetin and kaempferol on growth of HL-60 cells was assessed by MTT assay. The effect of quercetin and kaempferol on cell cycle in HL-60 cells was detected by flow cytometry. The cytotoxic effect of these 2 drugs was analysed by single cell electrophoresis assay. Western blot analysis was used to study the apoptotic mechanism of HL-60 cells. The results showed that the quercetin and kaempferol had a significant anti-leukemia effect in vitro. The proliferation of HL-60 cells was significantly inhibited in dose-and time-dependent manners after treating with quercetin (r = 0.77) and kaempferol (r = 0.76) respectively, and the cytotoxicity of quercetin was superior to that of kaempferol. The quercetin and kaempferol induced G(2)/M arrest and apoptosis of HL-60 cells. The quercetin and kaempferol could down-regulate the survivin expression. It is concluded that the quercetin and kaempferol have significant anti-leukemia effect in vitro. Furthermore the apoptosis-inducing effect of quercetin is stronger than that of kaempferol, both of which induce apoptosis of HL-60 cells through depressing cell growth, arresting cell cycle and inhibiting expression of survivin.

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PMID: 

Phytother Res. 2010 Mar ;24(3):419-23. PMID: 19827031

Abstract Title: 

Kaempferol protects HIT-T15 pancreatic beta cells from 2-deoxy-D-ribose-induced oxidative damage.

Abstract: 

During the progression of Type 2 diabetes, glucose toxicity is likely to contribute importantly to progressive beta cell failure. Oxidative stress is an important aspect of glucose toxicity in pancreatic beta cells, and reducing sugars, such as 2-deoxy-D-ribose (dRib), produce reactive oxygen species. Furthermore, many of the biological properties of flavonoids are likely to be related to their antioxidant and free-radical scavenging abilities. Accordingly, in the present study, we investigated whether kaempferol (a flavonol) protects beta cells from dRib-induced oxidative damage. HIT-T15 cells were cultured with various concentrations of dRib for 24h. Cell survivals, amounts of reactive oxygen species (ROS) generated, apoptosis, and lipid peroxidation were measured. dRib was found to dose-dependently reduce cell survival and to markedly increase intracellular ROS levels, apoptosis, and lipid peroxidation. However, kaempferol (10 microM) suppressed dRib (20 mM) induced intracellular ROS, apoptosis, and lipid peroxidation. So, we demonstrate that kaempferol reduces dRib-mediated beta cell damage interfering with ROS metabolism and protective effects against lipid peroxidation. Our findings indicate that kaempferol protects HIT-T15 cells from dRib-induced associated oxidative damage.

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PMID: 

Inflamm Res. 2010 Oct ;59(10):847-54. Epub 2010 Apr 13. PMID: 20383790

Abstract Title: 

Quercetin and kaempferol suppress immunoglobulin E-mediated allergic inflammation in RBL-2H3 and Caco-2 cells.

Abstract: 

OBJECTIVE: We investigated the inhibitory effects of quercetin and kaempferol treatment on the suppression of immunoglobulin E (IgE)-mediated allergic responses in relation to intestinal epithelium barrier function in RBL-2H3 and Caco-2 cells.METHODS: RBL-2H3 cells as a model of intestinal mucosa mast cells were treated with flavonols followed by IgE-anti-dinitrophenyl sensitization. The extent of degranulation and the release of pro-inflammatory cytokines were measured. Caco-2 cells were stimulated with interleukin (IL)-4 or IgE-allergen with or without flavonol pretreatment and changes in the expression of CD23 mRNA and mitogen-activated protein kinase (MAPK), and chemokine release were determined.RESULTS: Flavonols inhibited the secretion of allergic mediators in RBL-2H3 cells and suppressed the CD23 mRNA expression and p38 MAPK activation in IL-4 stimulated Caco-2 cells. Flavonols also suppressed IgE-OVA induced extra signal-regulated protein kinase (ERK) activation and chemokine release.CONCLUSIONS: Quercetin and kaempferol effectively suppressed the development of IgE-mediated allergic inflammation of intestinal cell models.

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PMID: 

In Vivo. 2005 Jan-Feb;19(1):69-76. PMID: 15796157

Abstract Title: 

Synergistic antiproliferative action of the flavonols quercetin and kaempferol in cultured human cancer cell lines.

Abstract: 

The consumption of vegetables containing the flavonols quercetin and kaempferol reduces the risk of cancer. We utilized human gut (HuTu-80 and Caco-2) and breast cancer cells (PMC42) to show the synergistic effect of quercetin and kaempferol in reducing cell proliferation. A trend in reduction of total cell counts was seen following a single exposure, a 4-day exposure or a 14-day exposure to quercetin and kaempferol. Combined treatments with quercetin and kaempferol were more effective than the additive effects of each flavonol. The reduction in cell proliferation was associated with decreased expression of nuclear proliferation antigen Ki67 and decreased total protein levels in treated cells relative to controls. In conclusion, the synergistic antiproliferative effect of quercetin and kaempferol demonstrated in cultured human cells has broad implications for understanding the influence of dietary nutrients in vivo, where anticancer effects may be a result of nutrients which act in concert.

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PMID: 

Naunyn Schmiedebergs Arch Pharmacol. 2019 Aug 2. Epub 2019 Aug 2. PMID: 31372697

Abstract Title: 

Sensitization of MDA-MBA231 breast cancer cell to docetaxel by myricetin loaded into biocompatible lipid nanoparticles via sub-G1 cell cycle arrest mechanism.

Abstract: 

The harmful dose-dependent side effects of chemotherapy drugs have caused the discovery of novel perspective to evaluate chemotherapy protocols. In this study, the potential application of Compritol was investigated as a major scaffold into nanostructured lipid careers to highlight myricetin efficiency in treatment of breast cancer cells along with codelivery of docetaxel (DXT). Characterization of myricetin-loaded NLCs was carried out by measuring the particle size and zeta potential, using the scanning electron microscopy. MTT, DAPI staining, flow cytometric, and RT-PCR (real-time) assays were used to recognize novel formulation behavior on cell cytotoxicity as well as recognizing molecular mechanism of formulation concerning apoptosis phenomenon. Myricetin-loaded NLCs reduced the cell viability from 50 ± 2.3 to 40 ± 1.3% (p 

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PMID: 

Mini Rev Med Chem. 2019 Oct 18. Epub 2019 Oct 18. PMID: 31648635

Abstract Title: 

Molecular Mechanisms of Action of Myricetin in Cancer.

Abstract: 

Natural compounds such as paclitaxel and camptothecin have great effects on tumor treating. Such natural chemicals often achieve anti-tumor effects through a variety of mechanisms. Therefore, it is of great significance to push forward the studies on the anticancer mechanism of natural anticancer to lay a solid foundation for the development of new drugs. Myricetin, originally isolated from Myrica nagi, is a natural pigment of flavonoids which can inhibit the growth of cancer cells (such as liver cancer, rectal cancer, skin cancer and lung cancer, etc.). It can regulate many intracellular activities (such as anti-inflammatory and blood lipids regulation) and can be even bacteriostatic. The purpose of this paper is to outline the molecular pathways of Myricetin's anticancer effects, including the effect on cancer cell death, proliferation, angiogenesis and metastasis and cell signaling pathway.

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PMID: 

J Pharm Pharmacol. 2019 Nov 14. Epub 2019 Nov 14. PMID: 31724745

Abstract Title: 

Potential of myricetin to restore the immune balance in dextran sulfate sodium-induced acute murine ulcerative colitis.

Abstract: 

OBJECTIVES: Myricetin is a bioactive compound in many edible plants with anti-inflammatory and anticarcinogenic activity. The current study aimed to determine the protective effects and mechanism of myricetin against ulcerative colitis (UC).METHODS: Myricetin was orally administered at doses of 40 and 80 mg/kg to C57BL/6 mice with UC induced using dextran sulfate sodium. The disease-associated index and colon length were determined at the end of the experiment, the proportion of Treg, Th1 and Th17 was analysed by cytometry, and cytokines were detected using ELISA.KEY FINDINGS: Myricetin (80 mg/kg) ameliorated the severity of inflammation in acute UC and significantly improved the condition. Myricetin (80 mg/kg) elevated the levels of IL-10 and transforming growth factor β. In addition, the proportion of regulatory T cells significantly increased in mice in the myricetin treatment group.CONCLUSIONS: Taking together, these results suggest that myricetin exhibits significant protective effects against UC and it could be used as a potential treatment for UC.

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PMID: 

Eur J Pharmacol. 2019 Nov 19:172805. Epub 2019 Nov 19. PMID: 31756333

Abstract Title: 

Possible osteoprotective effects of myricetin in STZ induced diabetic osteoporosis in rats.

Abstract: 

Myricetin is a flavonoid which has many pharmacological effects. However, to date there is no evidence study on the effect of myricetin in diabetic condition. This study was aimed to investigate whether myricetin could protect against diabetic osteoporosis in streptozotocin induced rats. Female Wistar rats were randomly allocated to four equal groups: diabetic group (DG), diabetic group with myricetin (50 mg per kilogram per day), (D) diabetic group with myricetin (100 mg/kg/day) and normal control group (CG). Body weight was recorded once a week. After treatment with myricetin for 12 weeks, serum biochemical analyses, the microarchitecture of femora, and histological changes were evaluated. Wefound that the bone mineral density (BMD) of myricetin (100 mg per kilogram per day)treatment group significantly increased than in the diabetic group (P 

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