Nanosecond electrical pulses may influence cells through mechanical stress.

PMID: 

PLoS One. 2016 ;11(5):e0154555. Epub 2016 May 2. PMID: 27135944

Abstract Title: 

Evaluation of the Genetic Response of U937 and Jurkat Cells to 10-Nanosecond Electrical Pulses (nsEP).

Abstract: 

Nanosecond electrical pulse (nsEP) exposure activates signaling pathways, produces oxidative stress, stimulates hormone secretion, causes cell swelling and induces apoptotic and necrotic death. The underlying biophysical connection(s) between these diverse cellular reactions and nsEP has yet to be elucidated. Using global genetic analysis, we evaluated how two commonly studied cell types, U937 and Jurkat, respond to nsEP exposure. We hypothesized that by studying the genetic response of the cells following exposure, we would gain direct insight into the stresses experienced by the cell and in turn better understand the biophysical interaction taking place during the exposure. Using Ingenuity Systems software, we found genes associated with cell growth, movement and development to be significantly up-regulated in both cell types 4 h post exposure to nsEP. In agreement with our hypothesis, we also found that both cell lines exhibit significant biological changes consistent with mechanical stress induction. These results advance nsEP research by providing strong evidence that the interaction of nsEPs with cells involves mechanical stress.

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This commentary criticizes the shortcomings of a study that found no connection between radiofrequency electromagnetic field exposure, cancers, and brain tumors.

PMID: 

Sci Total Environ. 2017 12 31 ;609:1. Epub 2017 Jul 26. PMID: 28732291

Abstract Title: 

Comments on"Radiofrequency electromagnetic fields and some cancers of unknown etiology: An ecological study".

Abstract: 

This correspondence refers to the Science of the Total Environment article by Gonzalez-Rubio et al. entitled"Radiofrequency electromagnetic fields and some cancers of unknown etiology: An ecological study". Authors of this paper have presented the findings of their preliminary epidemiological study which combined epidemiology, statistics and geographical information systems (GIS). Gonzalez-Rubio et al. have analyzed the possible link between exposure to Radiofrequency Electromagnetic Fields (RF-EMF) in the city of Albacete, Spain and the incidence of cancers such as lymphomas, and brain tumors. The shortcomings of this study are discussed.

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Transportation microenvironments are the largest source of exposure in European cities.

PMID: 

J Expo Sci Environ Epidemiol. 2018 03 ;28(2):147-160. Epub 2017 Aug 2. PMID: 28766560

Abstract Title: 

Radiofrequency electromagnetic field exposure in everyday microenvironments in Europe: A systematic literature review.

Abstract: 

The impact of the introduction and advancement in communication technology in recent years on exposure level of the population is largely unknown. The main aim of this study is to systematically review literature on the distribution of radiofrequency electromagnetic field (RF-EMF) exposure in the everyday environment in Europe and summarize key characteristics of various types of RF-EMF studies conducted in the European countries. We systematically searched the ISI Web of Science for relevant literature published between 1 January 2000 and 30 April 2015, which assessed RF-EMF exposure levels by any of the methods: spot measurements, personal measurement with trained researchers and personal measurement with volunteers. Twenty-one published studies met our eligibility criteria of which 10 were spot measurements studies, 5 were personal measurement studies with trained researchers (microenvironmental), 5 were personal measurement studies with volunteers and 1 was a mixed methods study combining data collected by volunteers and trained researchers. RF-EMF data included in the studies were collected between 2005 and 2013. The mean total RF-EMF exposure for spot measurements in European"Homes"and"Outdoor"microenvironments was 0.29 and 0.54 V/m, respectively. In the personal measurements studies with trained researchers, the mean total RF-EMF exposure was 0.24 V/m in"Home"and 0.76 V/m in"Outdoor". In the personal measurement studies with volunteers, the population weighted mean total RF-EMF exposure was 0.16 V/m in"Homes"and 0.20 V/m in"Outdoor". Among all European microenvironments in"Transportation", the highest mean total RF-EMF 1.96 V/m was found in trains of Belgium during 2007 where more than 95% of exposure was contributed by uplink. Typical RF-EMF exposure levels are substantially below regulatory limits. We found considerable differences between studies according to the type of measurements procedures, which precludes cross-country comparison or evaluating temporal trends. A comparable RF-EMF monitoring concept is needed to accurately identify typical RF-EMF exposure levels in the everyday environment.

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Extremely low frequency electromagnetic fields stimulates oxidative stress in human keratinocytes.

PMID: 

Biotechnol Appl Biochem. 2017 May ;64(3):415-422. Epub 2016 Oct 10. PMID: 27001710

Abstract Title: 

Effect of extremely low-frequency electromagnetic fields on antioxidant activity in the human keratinocyte cell line NCTC 2544.

Abstract: 

Some epidemiological studies have suggested possible associations between exposure to extremely low-frequency electromagnetic fields (ELF-EMFs) and various diseases. Recently, ELF-EMF has been considered as a therapeutic agent. To support ELF-EMF use in regenerative medicine, in particular in the treatment of skin injuries, we investigated whether significant cell damage occurs after ELF-EMF exposure. Reactive oxygen species (ROS) production was evaluated in the human keratinocyte exposed for 1 H to 50 Hz ELF-EMF in a range of field strengths from 0.25 to 2 G. Significant ROS increases resulted at 0.5 and 1 G and under these flux densities ROS production, glutathione content, antioxidant defense activity, and lipid peroxidation markers were assessed for different lengths of time. Analyzed parameters of antioxidant defense and membrane integrity showed a different trend at two selected magnetic fluxes, with a greater sensitivity of the cells exposed to 0.5 G, especially after 1 H. All significant alterations observed in the first 4 H of exposure reverted to controls 24 H after suggesting that under these conditions, ELF-EMF induces a slight oxidative stress that does not overwhelm the metabolic capacity of the cells or have a cytotoxic effect.

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Transcriptome signatures of p,p´-DDE-induced liver damage in Mus spretus mice.

PMID: 

Environ Pollut. 2018 Jul ;238:150-167. Epub 2018 Mar 20. PMID: 29554563

Abstract Title: 

Transcriptome signatures of p,p´-DDE-induced liver damage in Mus spretus mice.

Abstract: 

The use of DDT (1,1,1-trichloro-2,2-bis(p-chlorophenyl) ethane) in some countries, although regulated, is contributing to an increased worldwide risk of exposure to this organochlorine pesticide or its derivative p,p'-DDE [1,1-dichloro-2,2-bis(p-chlorophenyl) ethylene]. Many studies have associated p,p'-DDE exposure to type 2 diabetes, obesity and alterations of the reproductive system, but their molecular mechanisms of toxicity remain poorly understood. We have addressed this issue by using commercial microarrays based on probes for the entire Mus musculus genome to determine the hepatic transcriptional signatures of p,p'-DDE in the phylogenetically close mouse species Mus spretus. High-stringency hybridization conditions and analysis assured reliable results, which were also verified, in part, by qRT-PCR, immunoblotting and/or enzymatic activity. Our data linked 198 deregulated genes to mitochondrial dysfunction and perturbations of central signaling pathways (kinases, lipids, and retinoic acid) leading to enhanced lipogenesis and aerobic glycolysis, inflammation, cell proliferation and testosterone catabolism and excretion. Alterations of transcript levels of genes encoding enzymes involved in testosterone catabolism and excretion would explain the relationships established between p,p´-DDE exposure and reproductive disorders, obesity and diabetes. Further studies will help to fully understand the molecular basis of p,p´-DDE molecular toxicity in liver and reproductive organs, to identify effective exposure biomarkers and perhaps to design efficient p,p'-DDE exposure counteractive strategies.

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Dichlorodiphenyltrichloroethane technical mixture regulates cell cycle and apoptosis genes through the activation of CAR and ERα in mouse livers.

PMID: 

Toxicol Appl Pharmacol. 2013 Sep 1 ;271(2):137-43. Epub 2013 May 14. PMID: 23684557

Abstract Title: 

Dichlorodiphenyltrichloroethane technical mixture regulates cell cycle and apoptosis genes through the activation of CAR and ERα in mouse livers.

Abstract: 

Dichlorodiphenyltrichloroethane (DDT) is a widely used organochlorine pesticide and a xenoestrogen that promotes rodent hepatomegaly and tumours. A recent study has shown significant correlation between DDT serum concentration and liver cancer incidence in humans, but the underlying mechanisms remain elusive. We hypothesised that a mixture of DDT isomers could exert effects on the liver through pathways instead of classical ERs. The acute effects of a DDT mixture containing the two major isomers p,p'-DDT (85%) and o,p'-DDT (15%) on CAR and ERα receptors and their cell cycle and apoptosis target genes were studied in mouse livers. ChIP results demonstrated increased CAR and ERα recruitment to their specific target gene binding sites in response to the DDT mixture. The results of real-time RT-PCR were consistent with the ChIP data and demonstrated that the DDT was able to activate both CAR and ERα in mouse livers, leading to target gene transcriptional increases including Cyp2b10, Gadd45β, cMyc, Mdm2, Ccnd1, cFos and E2f1. Western blot analysis demonstrated increases in cell cycle progression proteins cMyc, Cyclin D1, CDK4 and E2f1 and anti-apoptosis proteins Mdm2 and Gadd45β. In addition, DDT exposure led to Rb phosphorylation. Increases in cell cycle progression and anti-apoptosis proteins were accompanied by a decrease in p53 content and its transcriptional activity. However, the DDT was unable to stimulate the β-catenin signalling pathway, which can play an important role in hepatocyte proliferation. Thus, our results indicate that DDT treatment may result in cell cycle progression and apoptosis inhibition through CAR- and ERα-mediated gene activation in mouse livers. These findings suggest that the proliferative and anti-apoptotic conditions induced by CAR and ERα activation may be important contributors to the early stages of hepatocarcinogenesis as produced by DDT in rodent livers.

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Epsom Salts Baths: A natural and effective muscle relaxant

Epsom Salt Bath salt in a wooden bowl with washcloths.In nature, there are some biological processes that are almost unstoppable. Once the process is started it creates a cascade of reactions and, like a freight train barreling down the track, stopping it takes an immense amount of effort.

One of the most classic of these, and perhaps the strongest in human biology, is labor. Once the signals arrive for labor to happen, they build on each other and become relentless. When the contractions start, those strong uterine contractions get stronger and stronger and build and come closer together. There are very few things that can stop contractions. But there are some very powerful medicines that can. One of these is called magnesium sulfate.

When it’s given it can calm even those powerful uterine contractions — slowing and even stopping them. (It is no longer one of the first-choice medicines, though, to delay pre-term labor). It is also powerful at calming the brain and preventing seizures in women with preeclampsia, where it remains the first-choice medicine. It’s on the WHO List of Essential Medicines.

What a lot of people don’t know is that Epsom salts are the same thing – magnesium sulfate. When you take an Epsom Salt bath, your body absorbs the magnesium and sulfate and this can cause real muscle relaxation.

Named after the town of Epsom, in Surrey England, it’s been used for hundreds of years. Despite generations who have reported relief, the absorption of magnesium through the skin has been controversial. It turns out that most of the magnesium is absorbed through the hair follicles.

When to Take an Epsom Salts Bath

Epsom Salt baths can be a great idea to deal with:

  • Leg cramps — Leg cramps are often worse when magnesium levels are a bit low.
  • Menstrual cramping – A warm bath by itself can help relax menstrual cramps. The addition of Epsom salts further relax muscle cramps and can provide additional relief.
  • Post-workout soreness – After a hard workout, and before bed, is a great time to take advantage of an Epsom Salt bath to reduce soreness the next day.
  • Chronic muscle pain – A recent randomized, controlled study found more relief when an Epsom salt, calcium bath was added to the treatment regimen.
  • A racing mind – Slipping in to a warm Epsom salts bath can be a signal to your brain that it’s time to let go and fully relax. This letting-go can be a significant sleep aid, as well.

In addition, you can get extra magnesium from soaking in an Epsom salts bath. Whole grains, legumes and dark-green leafy vegetables are good dietary sources of magnesium – but not all kids get enough that way.

Epsom Salts and Children

Epsom salts are gentle enough to be used for children, though I recommend waiting until they are six years old, unless you talk with your doctor. Children younger than six may be fine with Epsom salt baths, but their skin is especially permeable, and they may absorb more magnesium than they need. They may need a smaller dose.

Whether using Epsom salts in the bath or not, children should never be left unsupervised to take a bath. When ingested magnesium sulfate is a laxative so children shouldn’t drink the bathwater, but then, they should never drink bathwater (yuck!).

Parents of children with medical conditions should check with their doctors before using Epsom salts baths for their children.

A warm bath at the end of a hectic day may seem like an indulgence, but Epsom Salt baths are not just an indulgence, sweet idea, or a quaint old ritual. They are a natural and yet very powerful choice for relaxing your muscles and your mind.

Added Note: A paste made from Epsom Salt is often used as a “drawing paste” to help remove splinters. Simply moisten some with a bit of water, apply it generously over where the splinter is, leave it overnight, and in the morning the splinter may be at the surface and easy to pull out.

References

Permeation of topically applied magnesium ions through human skin is facilitated by hair follicles. Magnesium Research. June 2016.

Green exercise and mg-ca-SO4 thermal balneotherapy for treatment of non-specific chronic low back pain: a randomized controlled trial. BMC Musculoskeletal Disorders. May 2019.

Comprehensive nutritional and dietary intervention for autism spectrum disorder – a randomized, controlled 12-month trial. Nutrients. March 2018.

The post Epsom Salts Baths: A natural and effective muscle relaxant appeared first on DrGreene.com.

Full-Spectrum CBD vs. CBD Isolate

If you have shopped for CBD, you’ve probably seen products marked either “full-spectrum” or “isolate”. While these terms sound confusing, they simply refer to the variety ofcannabinoidd in the product.

Here is a breakdown of the differences between Full-Spectrum and Isolate CBD.

Full-Spectrum CBD is the most common variety you will find and the best option if you are looking for CBD to help with a variety of aliments. Full-Spectrum CDB products are made with multiple cannabinoids extracted from the hemp plant. Contrary to popular belief and the spread of misinformation, CBD does not contain TCH.
Tetrahydrocannabinol, or TCH is a psychoactive cannabinoid responsible for a marijuana high. Federal law requires all that CBD products be extracted from hemp and not marijuana plants. As a result, CDB only contains trace amounts of TCH that will not cause a high.

In short, Full-Spectrum CDB is a combination of different cannabinoids combined to work together for maximum benefits. A 2005 study conducted in Jerusalem showed that those given Full-Spectrum CBD “experienced heightened relief“ versus subjects given CBD isolate.

CBD isolate is the most basic form of CBD. Isolates do not contain other cannabinoids-it is just pure, isolated CBD from hemp. CBD isolates do not contain any additives such as flavoring.

While a CBD isolate might seem like the simpler of the two, it is much more difficult to extract only CBD from hemp and no other cannabinoids. During the hemp extraction process, all cannabinoids are removed. When making full-spectrum CBD, multiple cannabinoids are combined which is much easier than extracting a single cannabinoid.

CBD isolate is mostly used in vape pens, or “dabs” or “dabbing”. Vaping or dabbing CBD isolate is when compounds are vaporized on a hot nail and inhaled. CBD isolate vaping is similar to an e-cigarette, but you don’t have to use an e-cigarette or a liquid. CBD isolate dabs are available in powders, crystals, wax, resin, or a glass-like material called “shatter”. Isolate contains 0.0% TCH while Full-Spectrum contains 0.3% THC.

CBD isolate is not as commonly purchased or used as Full-Spectrum CBD products. Isolate is starting to show up more in CBD edibles as well as in oils. Like other forms of CBD, isolate may help with common health issues.

REDOX proteomics reveals energy metabolism alterations in the liver of M. spretus mice exposed to p, p’-DDE.

PMID: 

Chemosphere. 2017 Nov ;186:848-863. Epub 2017 Aug 13. PMID: 28826133

Abstract Title: 

REDOX proteomics reveals energy metabolism alterations in the liver of M. spretus mice exposed to p, p'-DDE.

Abstract: 

The toxicity induced by the pesticide 2,2-bis(p-chlorophenyl)-1,1,1,-trichloroethane (DDT) and its derivative 1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene (p,p'-DDE) has been associated with mitochondrial dysfunction, uncoupling of oxidative phosphorylation and respiratory chain electron transport, intracellular ion imbalance, generation of reactive oxygen species and impairment of the antioxidant defense system. A disruption in the cellular redox status causes protein Cys-based regulatory shifts that influence the activity of many proteins and trigger signal transduction alterations. Here, we analyzed the ability of p,p'-DDE to alter the activities of hepatic antioxidants and glycolytic enzymes to investigate the oxidative stress generation in the liver of p,p'-DDE-fed M. spretus mice. We also determined the consequences of the treatment on the redox status in the thiol Cys groups. The data indicate that the liver of p,p'-DDE exposed mice lacks certain protective enzymes, and p,p'-DDE caused a metabolic reprogramming that increased the glycolytic rate and disturbedthe metabolism of lipids. Our results suggested that the overall metabolism of the liver was altered because important signaling pathways are controlled by p,p'-DDE-deregulated proteins. The histological data support the proposed metabolic consequences of the p,p'-DDE exposure.

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The effect of acute dichlorodiphenyltrichloroethane exposure on hypermethylation status and down-regulation of p53 and p16INK4a genes in rat liver.

PMID: 

Environ Toxicol. 2016 May ;31(5):584-92. Epub 2014 Nov 20. PMID: 25410620

Abstract Title: 

The effect of acute dichlorodiphenyltrichloroethane exposure on hypermethylation status and down-regulation of p53 and p16INK4a genes in rat liver.

Abstract: 

The aim of the study was to investigate the early effect of acute dichlorodiphenyltrichloroethane (DDT) exposure on the methylation status of the promoter region of two tumor suppressor genes: p53 and p16(INK4a) (p16) in rat liver. We analyzed their transcript and protein expression profiles concurrently with the examination of transcriptional and protein expression levels of DNA (cytosine-5)-methyltransferase 1 (Dnmt1). Male Wistar rats were treated with a single dose of DDT (57 mg kg(-1) of body weight) and the methylation status of p53 and p16 genes was examined after 24 h using methylation-sensitive restriction analysis-MSRA. The obtained results indicate that DDT induced alternations in methylation of the promoter region in both p53 and p16 genes. In all the tested samples, the promoter CpG islands of p53 (-261, -179, and -450) were methylated within 100% as compared to control samples (0%). The methylation status of the p16 promoter (-11 and +77) was also altered due to exposure to DDT. Methylated cytosines were detectable in 75% of the tested DNA samples. The Real-time PCR and western blot analyses showed a decrease in mRNA and protein levels of p53, respectively, which was related to the increase in DNA synthesis. These relationships were also observed for mRNA and protein expressions of p16, although to a slighter extent. We also showed that hypermethylation in the promoter region of both tumor suppressor genes was consistent with an increased Dnmt1 mRNA level, and this relationship was further confirmed at the protein level of DNMT1. Concluding, our data suggests that epigenetically mediated changes in gene expression may play an important role in the mechanism of DDT toxicity, including carcinogenic action.

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