PMID: 

Med Gas Res. 2017 Apr-Jun;7(2):101-106. Epub 2017 Jun 30. PMID: 28744362

Abstract Title: 

Protective effects of hydrogen rich water on the intestinal ischemia/reperfusion injury due to intestinal intussusception in a rat model.

Abstract: 

This study aimed to investigate the protective effects of hydrogen rich water on the intestinal ischemia/reperfusion (I/R) injury in a rat intestinal intussusception (II) model. Ninety Sprague-Dawley rats were randomly assigned into three groups (= 30 per group). In sham group, rats received laparotomy, and the intestine was exposed for 15 minutes without II. In I/R + saline group and I/R + hydrogen group, rats received II after laparotomy and then intestine was relocated 8 hours later, followed by immediately intraperitoneal injection of normal saline and hydrogen rich water (HRW) (5 mL/kg), respectively. One hour later, the intestine was collected for hematoxylin-eosin staining and immunohistochemistry for apoptotic cells and 8-oxo-deoxyguanosine, and blood was harvested for detection of tumor necrosis factor-α, malondialdehyde and superoxide dismutase. Hematoxylin-eosin staining showed the intestinal mucosa was significantly damaged in I/R + saline group, which was markedly attenuated after HRW treatment. The serum tumor necrosis factor-α content increased significantly in I/R + saline group, but HRWtreatment reduced serum tumor necrosis factor-α content as compared to I/R + saline group (

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PMID: 

Mol Clin Oncol. 2017 Nov ;7(5):891-896. Epub 2017 Sep 1. PMID: 29142752

Abstract Title: 

Protective effect of hydrogen-rich water on liver function of colorectal cancer patients treated with mFOLFOX6 chemotherapy.

Abstract: 

The present study was conducted to investigate the protective effect of hydrogen-rich water on the liver function of colorectal cancer (CRC) patients treated with mFOLFOX6 chemotherapy. A controlled, randomized, single-blind clinical trial was designed. A total of 152 patients with CRC were recruited by the Department of Oncology of Taishan Hospital (Taian, China) between June 2010 and February 2016, among whom 146 met the inclusion criteria. Subsequently, 144 patients were randomized into the treatment (n=80) and placebo (n=64) groups. At the end of the study, 76 patients in the hydrogen treatment group and 60 patients in the placebo group were included in the final analysis. The changes in liver function after the chemotherapy, such as altered levels of alanine aminotransferase (ALT), aspartate transaminase (AST), alkaline phosphatase, indirect bilirubin (IBIL) and direct bilirubin, were observed. The damaging effects of the mFOLFOX6 chemotherapy on liver function were mainly represented by increased ALT, AST and IBIL levels. The hydrogen-rich water group exhibited no significant differences in liver function before and after treatment, whereas the placebo group exhibited significantly elevated levels of ALT, AST and IBIL. Thus, hydrogen-rich water appeared to alleviate the mFOLFOX6-related liver injury.

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PMID: 

Neurosci Lett. 2018 02 5 ;665:195-199. Epub 2017 Dec 6. PMID: 29223865

Abstract Title: 

Prevention of ischemia-induced hearing loss by intravenous administration of hydrogen-rich saline in gerbil.

Abstract: 

OBJECTIVE: Hydrogen-rich water, which is a potent antioxidant agent, was investigated for its protective effects against ischemic damage of the cochlea in gerbils.METHODS: The animals were subjected to transient cochlear ischemia by occluding the bilateral vertebral arteries for l5min. Five milliliters of hydrogen-rich saline was then intravenously administered immediately after the insult. Saline without hydrogen was used as a control. Effects of hydrogen were evaluated using the auditory brainstem response (ABR) and histological studies of the inner ear.RESULTS: In non-ischemia animals, ABR thresholds and histological findings of the cochlea did not change by administration of saline or hydrogen-rich saline. In contrast, transient cochlear ischemia caused a 24.2±3.8dB increase in the ABR threshold at 8kHz, and a decrease of 14.1%±1.8% in the number of inner hair cells (IHCs) at the basal turn on day 7. Ischemic damage was more severe at 16 and 32kHz. When the animals were treated with hydrogen-rich saline, cochlear damage was significantly reduced: the increase in ABR threshold was 11.7±2.6dB at 8kHz and the IHC loss was 7.5%±2.1% at the basal turn on day 7. The effects of hydrogen-rich saline were more prominent at higher frequencies.CONCLUSIONS: Intravenous administration of hydrogen-rich saline was effective in preventing acute hearing loss due to transient cochlear ischemia.

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PMID: 

Zhonghua Wei Zhong Bing Ji Jiu Yi Xue. 2018 Apr ;30(4):317-321. PMID: 29663991

Abstract Title: 

[Effect of hydrogen-rich water on the chondriosome damage and cytokines in brain tissue of rats with traumatic brain injury].

Abstract: 

OBJECTIVE: To observe the effect of hydrogen-rich water on the chondriosome damage and cytokines change in brain tissue of rats with traumatic brain injury (TBI).METHODS: Fifty-four health male Sprague-Dawley (SD) rats were divided into three groups by random number table: sham group, trauma group (TBI group), and trauma+hydrogen-rich water group (TBI+HW group), the rats in each group were subdivided into 1, 3 and 7 days subgroups according to the time points after trauma, with 6 rats in each subgroup. The TBI model was reproduced by using a modified Feency method for free fall impact, and the rats in sham group were not given brain impact after craniotomy. The rats in TBI+HW group were given intraperitoneal injection of hydrogen-rich water (5 mL/kg) after TBI model reproduction, and then once a day until being sacrificed; and the rats in sham group and TBI group were given the same amount of normal saline. The neurological severity scores (NSS) for neurologic deficits were calculated at corresponding time points, and then the rats were sacrificed to harvest brain tissue at 3 mm around lesion boundary. The cytokines including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) were determined by enzyme linked immunosorbent assay (ELISA); the protein expressions of Bax, Bcl-2 were determined by Western Blot; the RFU of reactive oxygen species (ROS), mitochondrial membrane potential (MMP) and mitochondrial membrane permeability (MPTP) were determined by fluorescence and enzyme sign method.RESULTS: TBI and TBI+HW groups appeared obvious neurologic damage after injury in rats. NSS scores in TBI and TBI+HW groups showed a decreased tendency with time prolongation after TBI. NSS scores in TBI+HW group at 3 days and 7 days were significantly lower than those of TBI group (NSS score: 9.67±0.82 vs. 11.17±1.17, 6.83±0.75 vs. 8.50±1.04, both P

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PMID: 

J Chem Neuroanat. 2018 09 ;91:55-62. Epub 2018 Apr 22. PMID: 29694843

Abstract Title: 

Structural and lipid peroxidation effects of lead on rat hippocampus and its attenuation by hydrogen rich water.

Abstract: 

Despite the well-known toxicity and the efforts to control its exposure, lead still has a serious health concern, particularly in young ages. Chelation therapy cannot correct the neurocognitive effects of chronic exposure. So, there is a requirement to test different protective agents for lead intoxication. Hydrogen-rich water (HRW) has gained attraction recently as an antioxidant. Four groups of rats received sodium acetate, HRW, lead acetate (LA), or LA plus HRW for 8 weeks. Oxidative stress, histological and immunohistochemistry using p53 antibody were used to investigate the toxic effect of lead and the possible HRW protective effect in rat hippocampus. Results showed that HRW corrected the elevated malondialdehyde levels (MDA) and restore the lead-induced depletion of antioxidant enzymes; glutathione reductase (GR), catalase (CAT) and superoxide dismutase (SOD). HRW does not affect the diminished nitric oxide (NO) level in the LA-treated group. Moreover, HRW reversed the LA-induced histological and immunohistochemical changes. It significantly decreased the percentage of the apoptotic index. We concluded that HRW protects the neurons against lead-induced oxidative stress and has anti-apoptotic effects without a noticeable change in NO level which already was diminished by LA.

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PMID: 

Free Radic Res. 2018 Dec ;52(11-12):1311-1322. Epub 2018 Apr 23. PMID: 29683360

Abstract Title: 

Hydrogen-rich water improves cognitive impairment gender-dependently in APP/PS1 mice without affecting Aβ clearance.

Abstract: 

Alzheimer's disease (AD) is characterised as a provoked inflammatory response and oxidative stress along with amyloidβ peptide (Aβ) deposition and neurofibrillary tangles in the brain, and effective treatment is greatly needed. Molecular hydrogen, which has been proposed to be an antioxidant that selectively reduces reactive oxygen species, was found to exert beneficial effects in Aβ injection-induced cognitivedysfunction. However, whether and how hydrogen affects AD pathogenesis remains uninvestigated. Thus, in the present study, APPswe/PS1dE9 (amyloid precursor protein (APP)/PS1) mice, a transgenic AD mouse model, were administered hydrogen-rich water for 3 months and the effects on cognitive functionand molecular pathways were investigated. We found that hydrogen-rich water significantly improved cognitive behaviour in female transgenic AD mice without affecting Aβ clearance, and reversed the brain oestrogen level, ERβ, and brain-derived neurotrophic factor (BDNF) expressions that were damaged in female transgenic AD mice, but not in males. Furthermore, hydrogen-rich water ameliorated oxidative stress and inflammatory responses more profoundly in the brains of female AD mice than in those of males. Our results demonstrate a novel sex-specific beneficial effect of hydrogen via oestrogenand brain ERβ-BDNF signalling in AD pathogenesis.

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PMID: 

J Lifestyle Med. 2019 Jan ;9(1):36-43. Epub 2019 Jan 31. PMID: 30918832

Abstract Title: 

Acute Supplementation with Molecular Hydrogen Benefits Submaximal Exercise Indices. Randomized, Double-Blinded, Placebo-Controlled Crossover Pilot Study.

Abstract: 

Background: Clinical studies have reported hydrogen-rich water (HRW) to have therapeutic and ergogenic effects. The aim of this study was to determine the effect of acute supplementation with HRW on exercise performance as measured by VO, respiratory exchange ratio (RER), heart rate (HR), and respiratory rate (RR).Methods: Baseline levels of all exercise indices were determined in nineteen (4 female, 23.4± 9.1 yr; 15 male, 30.5 ± 6.8 yr) healthy subjects using a graded treadmill exercise test to exhaustion. Each subject was examined two additional times in a randomized double-blinded, placebo-controlled crossover fashion. Subjects received either HRW or placebo, which was consumed the day before and the day of the testing. HRW was delivered using the hydrogen-producing tablets, DrinkHRW (5 mg of H). All data was analyzed with SPSS using pairwise comparisons with Bonferroni adjustment.Results: HRW supplementation did not influence maximal or minimal indices of exercise performance (VO, RER, HR and RR) (p

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PMID: 

Curr Mol Med. 2019 ;19(4):294-302. PMID: 30907314

Abstract Title: 

Attenuation of Cardiac Ischaemia-reperfusion Injury by Treatment with Hydrogen-rich Water.

Abstract: 

BACKGROUND: Hydrogen has been shown to exert a bioactive effect on the myocardium. This study examined the signalling pathways for hydrogen attenuating ischaemia-reperfusion injury.METHODS: In total, 20 male Wistar rats were evaluated for the effects of hydrogen-rich water on ischaemia-reperfusion in hearts. Left ventricular tissue was taken for screening and analysis of active protein factors by protein chip technology. The enrichment of the KEGG pathway was obtained by using the Gene Ontology (GO) enrichment principle. The expression of JAK2, STAT1, STAT3, p-STAT1, p-JAK2, p-STAT3 in rat myocardium was detected by Western blot analysis and immunohistochemistry. The apoptosis rates of the control and hydrogen-rich water groups were detected by TUNEL staining.RESULTS: The expression levels of 25 proteins, including five transduction pathways, were downregulated in the hydrogen-rich water group. The expression levels of p- JAK2/JAK2, p-STAT3/STAT3 were upregulated in the hydrogen-rich water group compared with the control group, and p-STAT1/STAT1 was downregulated in the hydrogen-rich water group compared with the control group. Furthermore, the apoptosis rate was significantly decreased in the hydrogen-rich water group, as well.CONCLUSION: Hydrogen-rich water may inhibit the apoptosis of cardiomyocytes after ischaemia-reperfusion by upregulating the expression of the JAK2-STAT3 signalling pathway, which reduces ischaemia-reperfusion injury.

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PMID: 

Clin Res Hepatol Gastroenterol. 2019 Apr 11. Epub 2019 Apr 11. PMID: 30982748

Abstract Title: 

Hydrogen-rich water reduces liver fat accumulation and improves liver enzyme profiles in patients with non-alcoholic fatty liver disease: a randomized controlled pilot trial.

Abstract: 

BACKGROUND AND AIMS: While non-alcoholic fatty liver disease (NAFLD) is rapidly becoming the most common liver disease worldwide, its treatment remains elusive. Since metabolic impairment plays a major role in NAFLD pathogenesis, any pharmaceuticals, such as molecular hydrogen (H), that advance lipid and glucose metabolism could be appropriate to tackle this complex condition. The aim of this study was to analyze the effects of 28-day hydrogen-rich water intake on liver fat deposition, body composition and lab chemistry profiles in overweight patients suffering from mild-to-moderate NAFLD.METHODS: Twelve overweight outpatients with NAFLD (age 56.2 ± 10.0 years; body mass index 37.7 ± 5.3 kg/m; 7 women and 5 men) voluntarily participated in this double-blind, placebo-controlled, crossover trial. All patients were allocated to receive either 1 L per day of hydrogen-rich water (HRW) or placebo water for 28 days. The study was registered at ClinicalTrials.gov (ID NCT03625362).RESULTS: Dual-echo MRI revealed that HRW significantly reduced liver fat accumulation in individual liver regions-of-interest at 28-day follow-up, as compared to placebo administration (P 

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PMID: 

Shanghai Kou Qiang Yi Xue. 2018 Jun ;27(3):252-256. PMID: 30411118

Abstract Title: 

[Hydrogen rich water attenuates pregnancy gingivitis induced by ligation in SD rats].

Abstract: 

PURPOSE: To investigate the effect of hydrogen rich water on experimental gingivitis in SD rats during pregnancy.METHODS: Female SD rats mated with male ones were chosen to induce experimental gingivitis after ligation for 2 weeks. The pregnant rats were randomly divided into control group, model group and HW group. In the control and model group, rats were given pure water, while animals in the HW group were given hydrogen-rich water twice a day. All pregnant animals were sacrificed on day 16 of pregnancy. The level of Prog, SOD and TNF-α in the gingiva of different groups were measured by ELISA, the expression of PR, NFκB and TNF-α were determined by immunohistochemistry and Western blot. SPSS 13.0 software package was used for data analysis.RESULTS: Pregnancy gingivitis of SD rats could be induced by thread ligation. PR was mainly distributed in the gingival epithelium, while there was no significant difference of Prog and PR in the gingiva among different groups(P>0.05). Furthermore, in the model group, lower SOD level as well as higher NFκB and TNF-α level were found in the gingiva. Compared with the model group, the inflammatory response of pregnancy gingivitis in HW group was significantly suppressed along with decreased NFκB and TNF-α.CONCLUSIONS: Progesterone and its receptor may play an indirect role in the process of pregnancy gingivitis of rats. Hydrogen rich water may be beneficial in suppressing pregnancy gingivitis progress by decreasing inflammatory response related to gingival oxidative stress.

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