PMID:
J Agric Food Chem. 2019 Dec 18. Epub 2019 Dec 18. PMID: 31850758
Abstract Title:
Protective Effect of Naringin on in vitro Gut-Vascular Barrier Disruption of Intestinal Microvascular Endothelial Cells Induced by TNF-α.
Abstract:
Naringin is a polymethoxylated flavonoid commonly found in citrus species, and has the therapeutic potential in intestinal disorder. However, the effect and mechanism of naringin on gut-vascular barrier disruption has not yet been reported. This study aimed to investigate the distinguishingly and selectively protective effects of naringin on TNF-α-induced gut-vascular barrier disruption and elucidate the potential mechanism. In the present study, an in vitro gut-vascular barrier model composed of rat intestinal microvascular endothelial cells (RIMVECs) was conducted. Evans blue-albumin efflux assay showed that naringin (50 μM) evidently protected the intergrity of RIMVECs monolayer barriers against TNF-α-induced disruption. Naringin maintained the expression and distribution of tight junction proteins including zona occludin-1, occludin, claudin-1 and claudin-2. Additionally, naringin protected RIMVECs from TNF-α-induced apoptosisand cell migration suppression (41.1 ± 2.2% vs 51.1 ± 3.5%; 61.0 ± 5.1% vs 72.2 ± 6.2%). Our results indicate that naringin effectively ameliorates gut-vascular barrier disruption.
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