PMID:
Wei Sheng Yan Jiu. 2019 Nov ;48(6):964-975. PMID: 31875823
Abstract Title:
[The role of GSK3β in adipose tissue inflammation induced by bisphenol-A in high fat diet fed mice].
Abstract:
OBJECTIVE: To study the effect of glycogen synthase kinase 3β(GSK3β) in BPA-induced adipose tissue inflammation in high fat diet(HFD) fed mice.METHODS: Four-week-old male C57 BL/6 mice were randomly divided into normal diet(ND) group, HFD group, HFD + GSK3β inhibitor group, HFD + 1000 nmol/L BPA group, HFD+1000 nmol/L BPA+GSK3β inhibitor group. The mice were exposed to BPA via drinking water. From the 14 th week of BPA exposure to the end of 16 weeks, the GSK3β inhibitor group was intraperitoneally injected with 21. 5 mg/kg lithium chloride(Li Cl)every two days for a total of 10 times. At the end of 16 weeks, the mice were sacrificed after anesthesia, and the epididymal fat tissue was taken aseptically. The pathological changes were observed by H&E staining. The expressions of interleukin-1β(IL-1β) and tumor necrosis factor-α(TNF-α) were detected by immunohistochemistry(IHC). The expression of GSK3β protein and its S9 serine(GSK3β-S9) phosphorylation were detected by western blot.RESULTS: Compared with the ND group, the body weight [(34. 97±1. 91) g]and epididymal fat pad coefficient [(3. 25±0. 39) %]of HFD group was significantly up-regulated(P
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