PMID:
Nan Fang Yi Ke Da Xue Xue Bao. 2019 Oct 30 ;39(10):1221-1226. PMID: 31801706
Abstract Title:
[Protective effect ofagainst oxidative stress in neonatal mice with necrotizing enterocolitis].
Abstract:
OBJECTIVE: To investigate the protective effect ofDSM17938 strain against oxidative stress in a neonatal mouse model of necrotizing enterocolitis (NEC) and explore the possible mechanism.METHODS: Ninety-six 10-day-old neonatal C57BL/6J mice were equally randomized into control group, NEC group, and NEC+group. The pathological changes of the ileocecal intestinal tissue were evaluated with HE staining and double-blind pathological scoring. The mRNA and protein expressions of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in the intestinal tissues were detected using quantitative real-time PCR and ELISA, respectively. Colorimetric assays were used to determine the activity of superoxide dismutase (SOD) and its inhibition rate, malondialdehyde (MDA), glutathione (GSH), oxidizedglutathione (GSSG), and GSSG/ GSH ratio.RESULTS: Compared with those in the control group, the neonatal mice in NEC group showed significant weight loss (
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