PMID:
Cancer Manag Res. 2020 ;12:1199-1209. Epub 2020 Feb 17. PMID: 32110094
Abstract Title:
Heavy Water Affects Vital Parameters of Human Melanoma Cells in vitro.
Abstract:
Purpose: Although regular water is composed of two hydrogens and one oxygen, referred to as HO, a small amount of water on this planet contains alternative forms of elements with different molecular weights because of the addition of neutrons. The present study was dedicated to studying the effect of heavy water (DO), in which the two hydrogens become substituted by deuterium, on the cell physiology of different human cells with particular focus on malignant melanoma cells.Methods: Cells were cultured in regular medium in which the content of HO was gradually substituted by DO or deuterium-depleted water (DDW). Following this, the changes of basic cellular parameters, such as morphology, migration, proliferation, cell cycle, apoptosis and microtubule integrity were examined.Results: It was found that raising the DO content above the standard levels led to a concentration-dependent decrease in proliferation. Lowering the DO levels below this level had no effect. Likewise, elevated DO levels hampered migration. Moreover, cell-cycle analysis showed an increase of sub-G1 cells. Corroboratively, markers for apoptosis were induced (histone-associated DNA fragments, Bax, and PARP). In regard to microtubule integrity, only very high levels of DO (75%) caused partial filament condensation.Conclusion: DO, although chemically identical with HO, shows proapoptotic and antiproliferative effects on melanoma cells. These findings give a closer look of this interesting compound.
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