Author Archives: Taylor Mercado

PMID:  J Vet Med Sci. 1994 Oct ;56(5):961-4. PMID: 7865600 Abstract Title:  Comparative analysis of the 5' non-coding region of pestivirus RNA detected from live virus vaccines. Abstract:  Comparative analysis of nucleotide sequences in the 5' non-coding region (NCR) of pestivirus RNA detected from live porcine and human virus vaccines indicated that the contaminants are …

Continue reading “Further evidence of pestivirus RNA detected in live virus vaccines.”

PMID:  Vaccine. 1995 Jan ;13(1):100-3. PMID: 7762264 Abstract Title:  Adventitious pestivirus RNA in live virus vaccines against bovine and swine diseases. Abstract:  Live virus vaccines against bovine and porcine diseases were examined for the presence of adventitious pestivirus RNA or pestiviruses by reverse transcription-polymerase chain reaction (PCR). Pestivirus RNA was detected in the live virus …

Continue reading “Fetal bovine serum used to grow the host cells used to prepare the bovine and swine viral vaccines can contaminate live virus vaccines.”

PMID:  Microbiol Immunol. 1995 ;39(12):979-85. PMID: 8789057 Abstract Title:  Demonstration and genotyping of pestivirus RNA from mammalian cell lines. Abstract:  We examined 20 cell lines of various animal origins for the presence of pestivirus contamination by the reverse transcription-polymerase chain reaction (RT-PCR), and found 15 (75%) cell lines were positive. The RT-PCR products of the …

Continue reading “Cell lines of bovine, canine, feline, and primate origin were shown to be contaminated with various genotypes of bovine diarrhea virus whereas cell lines of caprine origin were contaminated with border disease virus.”

PMID:  Vet Ital. 2004 Jan-Mar;40(1):7-21. PMID: 20437384 Abstract Title:  Genotypes of Pestivirus RNA detected in anti-influenza virus vaccines for human use. Abstract:  Nine polyvalent human influenza virus vaccines were tested by reverse transcriptase-polymerase chain reaction (RT-PCR) for the presence of pestivirus RNA. Samples were selected from manufacturers in Europe and the USA. Three samples of …

Continue reading “33% of influenza vaccines tested gave positive results for pestivirus RNA, belonging to the bovine viral diarrhea virus species.”

PMID:  Mol Cell Probes. 2008 Jun ;22(3):212-4. Epub 2008 Feb 16. PMID: 18395415 Abstract Title:  Simple procedures to obtain exogenous internal controls for use in RT-PCR detection of bovine pestiviruses. Abstract:  Pestiviruses are ubiquitous pathogens of cattle and frequent adventitious viruses in biologicals. Furthermore, it has been suggested that these agents might be related to …

Continue reading ““Pestiviruses are ubiquitous pathogens of cattle and frequent adventitious viruses in biologicals. Furthermore, it has been suggested that these agents might be related to infantile gastroenteritis and microencephaly.””

PMID:  Biologicals. 2000 Mar ;28(1):41-6. PMID: 10799055 Abstract Title:  Evaluation of vaccines, interferons and cell substrates for pestivirus contamination. Abstract:  Pestiviruses are potential contaminants of biological products produced in bovine or porcine cells or manufactured via processes using animal-derived raw materials such as bovine serum. In order to investigate possible contamination of products including those …

Continue reading “Cell banks derived from non-human primate, hamster or rabbit kidney cell lines, or cultures of primary chick embryo fibroblasts, may be infected with BVDV if exposed to pestivirus contaminated raw materials during manufacture.”

PMID:  Biologicals. 2010 May ;38(3):332-4. Epub 2010 Apr 24. PMID: 20456974 Abstract Title:  Human and animal vaccine contaminations. Abstract:  Vaccination is one of the most important public health accomplishments. However, since vaccine preparation involves the use of materials of biological origin, vaccines are subject to contamination by micro-organisms. In fact, vaccine contamination has occurred; a …

Continue reading “New techniques for vaccine virus production has lead to a reduced risk of vaccine contamination, but has not eliminated that risk.”

PMID:  N Engl J Med. 2011 Jun 2 ;364(22):2101-10. PMID: 21631322 Abstract Title:  Early-childhood membranous nephropathy due to cationic bovine serum albumin. Abstract:  BACKGROUND: The M-type phospholipase A(2) receptor (PLA(2)R) was recently identified as a candidate antigen in 70% of cases of idiopathic membranous nephropathy, a common form of the nephrotic syndrome. The nature of …

Continue reading “Some patients with childhood membranous nephropathy have both circulating cationic bovine serum albumin and anti-bovine serum albumin antibodies, of which is present in vaccines.”

PMID:  Vaccine. 2016 12 12 ;34(51):6617-6625. Epub 2016 Jun 15. PMID: 27317264 Abstract Title:  Adventitious agents and live viral vectored vaccines: Considerations for archiving samples of biological materials for retrospective analysis. Abstract:  Vaccines are one of the most effective public health medicinal products with an excellent safety record. As vaccines are produced using biological materials, …

Continue reading “Vaccines have a history of being contaminated with adventitious agents like porcine circovirus 1, simian virus 40, bacteriophage, avian retrovirus, and more.”

PMID:  Vaccine. 2017 03 13 ;35(11):1494-1500. Epub 2017 Feb 16. PMID: 28216185 Abstract Title:  Sensitization to bovine serum albumin as a possible cause of allergic reactions to vaccines. Abstract:  BACKGROUND: Immediate type hypersensitivity to vaccines containing bovine/porcine excipients, such as the measles, mumps and rubella (MMR) vaccine is probably due to sensitization to bovine/porcine gelatin. …

Continue reading “Sensitization to bovine serum albumin is possibly playing a role in allergic reactions to vaccines.”